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Anti-ELAVL1 / HUR Antibody LS-C7451

Ordering

Wt. Vol. Conc. Price
- 1000 µl - $515
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Mouse Monoclonal (IgG1,k) to Human ELAVL1 / HUR
Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human ELAVL1 / HUR
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal
Tissue culture supernatant

Applications

IHC - Paraffin (0.1 - 1 µg/ml), Immunofluorescence (1 - 5 µg/ml), Western blot (1 - 5 µg/ml), Immunoprecipitation (5 - 10 µg/ml), ELISA (0.1 - 1 µg/ml)

Specificity and Use

ELAVL1 / HUR antibody was raised against recombinant human HuR protein.
Recognizes human HuR, HuB, HuC and HuD proteins at ~34-36kD.
Suitable for use in Western Blot, ELISA, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Western Blot: 1-5 ug/ml. ELISA: 0.1-1 ug/ml. Immunoprecipitation (Native): 5-10 ug/ml. Immunofluorescence: 1-5 ug/ml. Immunohistochemistry: 0.1-1.0 ug/ml; For paraffin-embedded tissues, heat induced epitope retrieval (HIER) using citrate buffer in a pressure cooker for 5 minutes is recommended. Positive control: WB: Human OVCAR-3, SKOV-3, CAOV-3, ES-2, PA-1, OAW42, Mdah2774, PA1, A27/80, EFO-27, and HeLa cells and human testis, spleen, skeletal muscle, lung, liver, kidney, heart, and brain homogenates. IP/IF: HeLa cells. IHC: Ovarian carcinoma tissues.

Packaging

0.1% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About ELAVL1 / HUR

Q15717 NM_001419 NP_001410.2

ELAVL1 Antibody, ELAV1 Antibody, Hua Antibody, HUR Antibody, Hu antigen R Antibody, ELAV-like protein 1 Antibody, Hu-antigen R Antibody, MelG Antibody

RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs.

Requested From: United States
Date Requested: 9/30/2016

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