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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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ELAC1 Antibody (aa180‑229) LS‑C101912
ELAC1 antibody LS-C101912 is an unconjugated rabbit polyclonal antibody to ELAC1 from human, rat, bat and other species. Validated for WB.
Catalog
Size
Price
LS-C101912-50
50 µg
Unavailable

Popular ELAC1 Products

Antibody
Species: Human
Applications: ICC, Western blot
Western blot of immunized recombinant protein using ELAC1 antibody.
Species: Human
Applications: ICC, Dot Blot
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Applications: Western blot, ELISA
Antibody
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Applications: Western blot, ELISA
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-ELAC1 mAb.
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Dot Blot

Product Description

ELAC1 antibody LS-C101912 is an unconjugated rabbit polyclonal antibody to ELAC1 from human, rat, bat and other species. Validated for WB.
About ELAC1
Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA. Q9H777 NM_018696 NP_061166.1

ELAC1 Antibody, D29 Antibody, Deleted in Ma29 Antibody, ElaC (E. coli) homolog 1 Antibody, ElaC homolog 1 (E. coli) Antibody, TRNA 3 endonuclease 1 Antibody, TRNA Z (short form) Antibody, Ribonuclease Z 1 Antibody, TRNase Z 1 Antibody, ElaC homolog protein 1 Antibody, RNase Z 1 Antibody, TRNase ZS Antibody


Specifications

Target
Human ELAC1
Host
Rabbit
Reactivity
Human, Monkey, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Mouse, Hamster (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
ELAC1 antibody was raised against synthetic peptide located between aa180-229 of human ELAC1 (Q9H777, NP_061166). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%); Mouse, Hamster (92%); Opossum, Zebra finch, Platypus (85%); Rat (84%).
Blocking Peptide
LS-E15218 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C101912. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa180-229
Specificity
Human ELAC1
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

ELAC1 antibody LS-C101912 Western blot of Fetal Heart lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ELAC1 antibody LS-C101912 Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ELAC1 antibody LS-C101912 Western blot of Fetal Heart lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ELAC1 antibody LS-C101912 Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ELAC1 antibody LS-C101912 Western blot of Fetal Heart lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ELAC1 antibody LS-C101912 Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ELAC1 antibody LS-C101912 Western blot of Fetal Heart lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ELAC1 antibody LS-C101912 Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/21/2018

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