(tested or 100% immunogen sequence identity)
EIF5A2 antibody was raised against a synthetic peptide representing the C-terminus of the Human EIF5 A2 protein. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Dog, Horse, Opossum (100%); Lizard (93%); Turkey, Chicken (80%).
Reacts with the human EIF5 A2 protein. Cross-reactivity with human eIF5 A1 protein has been shown in endogenous samples.
Immunohistochemistry: LS-B281 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B281 was determined to be 5 ug/ml.
4°C or -20°C, Avoid freeze-thaw cycles.
This antibody carries the LSBio 100% Guarantee.