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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EIF5A2 Antibody (aa139‑153) IHC‑plus™ LS‑B281 Best Seller
Note: This antibody replaces LS-C2657, LS-C65786, LS-C65785
EIF5A2 antibody LS-B281 is an unconjugated rabbit polyclonal antibody to human EIF5A2. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B281-50
50 µg (1 mg/ml)
Unavailable

Popular EIF5A2 Products

Antibody
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Product Description

EIF5A2 antibody LS-B281 is an unconjugated rabbit polyclonal antibody to human EIF5A2. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
About EIF5A2
mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. Q9GZV4 NM_020390 NP_065123.1

EIF5A2 Antibody, EIF-5A-2 Antibody, EIF-5A2 Antibody, EIF5AII Antibody


Specifications

Target
Human EIF5A2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
EIF5A2 antibody was raised against a synthetic peptide representing the C-terminus of the Human EIF5 A2 protein. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Dog, Horse, Opossum (100%); Lizard (93%); Turkey, Chicken (80%).
Epitope
aa139-153
Specificity
Reacts with the human EIF5 A2 protein. Cross-reactivity with human eIF5 A1 protein has been shown in endogenous samples.
Usage
Immunohistochemistry: LS-B281 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B281 was determined to be 5 ug/ml.
Presentation
PBS, 0.02% sodium azide
Storage
4°C or -20°C, Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Anti-eIF5A2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B281 concentration 5 ug/ml.
Anti-eIF5A2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B281 concentration 5 ug/ml.

Immunohistochemistry

Anti-eIF5A2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B281 concentration 5 ug/ml.
Anti-eIF5A2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B281 concentration 5 ug/ml.

Immunohistochemistry

Anti-eIF5A2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B281 concentration 5 ug/ml.
Anti-eIF5A2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B281 concentration 5 ug/ml.

Immunohistochemistry

Anti-eIF5A2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B281 concentration 5 ug/ml.
Anti-eIF5A2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B281 concentration 5 ug/ml.

Requested From: 
Date Requested: 7/15/2018

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