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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EIF4E3 Antibody (C‑Terminus) LS‑C82443 Best Seller
EIF4E3 antibody LS-C82443 is an unconjugated rabbit polyclonal antibody to EIF4E3 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C82443-50
50 µg
Unavailable

Popular EIF4E3 Products

Western blot of extracts from LOVO cells, using EIF4E3 Antibody. The lane on the right is treated with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human, Mouse, Rat
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Western blot of eIF4E3 antibody
Species: Human, Mouse, Rat
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Immunofluorescence, Western blot, ELISA

Product Description

EIF4E3 antibody LS-C82443 is an unconjugated rabbit polyclonal antibody to EIF4E3 from human, mouse, rat and other species. Validated for WB.
About EIF4E3
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis. May act as an inhibitor of EIF4E1 activity. Q8N5X7 Q8N5X7

EIF4E3 Antibody, EIF4E type 3 Antibody, EIF-4E3 Antibody, EIF4E-3 Antibody, EIF-4E type 3 Antibody


Specifications

Target
Human EIF4E3
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
EIF4E3 antibody was raised against synthetic peptide from C-Terminus of human EIF4E3 (Q8N5X7, NP_001128123). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Xenopus, Catfish, Salmon, Smelt, Zebrafish (100%); Turkey, Chicken, Platypus, Lizard (92%); Seabass (85%).
Blocking Peptide
LS-E15126 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C82443. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human EIF4E3
Usage
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 0.5 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Fetal liver cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 15%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal liver cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 15%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal liver cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 15%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal liver cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 15%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal liver cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 15%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal liver cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 15%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal liver cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 15%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal liver cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 15%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

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