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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EIF4E Antibody (phospho‑Ser209) LS‑C9777
Antibody
EIF4E antibody LS-C9777 is an unconjugated rabbit polyclonal antibody to EIF4E from human, mouse and rat. Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C9777-100
100 µl
Unavailable

Popular EIF4E Products

Antibody
Species: Human, Mouse, Rat
Applications: Western blot, ELISA
Dot blot of anti-EIF4E-pS209 Phospho-specific antibody (RB13353) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Species: Human
Applications: Dot Blot
Anti-EIF4E antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6817 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: IHC, Western blot, Flow Cytometry, ELISA
Antibody
Species: Human
Applications: ICC, Immunofluorescence, Western blot, ELISA

Product Description

EIF4E antibody LS-C9777 is an unconjugated rabbit polyclonal antibody to EIF4E from human, mouse and rat. Validated for ELISA, IHC and WB.
About EIF4E
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. P06730 NM_001968 NP_001959.1

EIF4E Antibody, EIF-4E Antibody, EIF4E1 Antibody, EIF4EL1 Antibody, EIF4F Antibody, CBP Antibody, EIF-4F 25 kDa subunit Antibody, MRNA cap-binding protein Antibody


Specifications

Target
Human EIF4E
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • Western blot (1:1000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
EIF4E antibody was raised against synthetic peptide (KLH coupled) corresponding to residues around Ser209 of eIF4E.
Epitope
pSer209
Specificity
Recognizes endogenous levels of total human eIF4E protein. The antibody does not cross-react with other proteins. Species cross-reactivity: mouse, rat, monkey and zebra fish.
Usage
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Western Blot: 1:1000. Immunohistochemistry (Paraffin and Frozen): 1:100.
Presentation
10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol. No preservative added.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/25/2018

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