Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
EIF2 is a translation initiation factor that catalyzes the first regulated step of protein synthesis, promoting the binding of the initiator tRNA to 40s ribosomal subunits. eIF2 is composed of 3 non identical subunits, alpha, beta and gamma. Binding occurs as a ternary complex of methionyl-tRNA, eIF2 and GTP. The rate of complex formation is modulated by the phosphorylation state of eIF2alpha. Phosphorylation of eIF2alpha has been shown to occur in response to heat shock and hypoxic stress.
Immunofluorescence of monoclonal antibody to EIF2S1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of EIF2S1 expression in transfected 293T cell line by EIF2S1 monoclonal antibody (M01), clone 3H12-C11.Lane 1: EIF2S1 transfected lysate(36.1 KDa).Lane 2: Non-transfected lysate.
EIF2S1 monoclonal antibody (M01), clone 3H12-C11 Western Blot analysis of EIF2S1 expression in HeLa.
EIF2S1 monoclonal antibody (M01), clone 3H12-C11. Western Blot analysis of EIF2S1 expression in HeLa NE.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged EIF2S1 is approximately 0.3 ng/ml as a capture antibody.
Western blot analysis of EIF2S1 over-expressed 293 cell line, cotransfected with EIF2S1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with EIF2S1 monoclonal antibody (M01), clone 3H12-C11 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Requested From: United States
Date Requested: 1/21/2017