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EGLN2 / PHD1 Antibody (C‑Terminus) LS‑C110437
PHD1 antibody LS-C110437 is an unconjugated rabbit polyclonal antibody to PHD1 (EGLN2) from human, mouse, rat and other species. Validated for WB.
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Species:
Human, Bovine, Guinea pig
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Species:
Rat, Human, Monkey, Mouse, Bovine, Dog, Guinea pig
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, Immunofluorescence, Western blot, Flow Cytometry
Product Description
PHD1 antibody LS-C110437 is an unconjugated rabbit polyclonal antibody to PHD1 (EGLN2) from human, mouse, rat and other species. Validated for WB.
About EGLN2 / PHD1
Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A.
Q96KS0
NM_017555
NP_444274.1
EGLN2 Antibody, EIT6 Antibody, EGL nine (C.elegans) homolog 2 Antibody, Egl nine homolog 2 Antibody, HPH-1 Antibody, HPH-3 Antibody, Estrogen-induced tag 6 Antibody, PHD1 Antibody, HIF-PH1 Antibody, HIF-prolyl hydroxylase 1 Antibody, HIFPH1 Antibody
Specifications
Target
Human EGLN2 / PHD1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Guinea pig
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
Immunogen
EGLN2 / PHD1 antibody was raised against synthetic peptide from C-Terminus of human EGLN2 (Q96KS0, NP_444274). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Mouse, Rat, Guinea pig (100%); Monkey, Marmoset, Elephant (85%); Dog (84%).
Blocking Peptide
LS-E14429 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110437. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunizing peptide used to generate LS-C110437. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human EGLN2 / PHD1
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images
Western blot
EGLN2 / PHD1 antibody LS-C110437 Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
EGLN2 / PHD1 antibody LS-C110437 Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
EGLN2 / PHD1 antibody LS-C110437 Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
EGLN2 / PHD1 antibody LS-C110437 Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From:
Date Requested: 6/20/2018
Date Requested: 6/20/2018
