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Anti-EGLN2 / PHD1 Antibody (C-Terminus) LS-C110437

Catalog	Size	Price
LS-C110437-50	50 µg	Unavailable

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Antibody:	Rabbit Monoclonal to Human EGLN2 / PHD1
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Antibody:	Rabbit Polyclonal to Human EGLN2 / PHD1
Reactivity:	Human, Mouse, Rat, Dog, Guinea pig
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

Details

Target:	Human EGLN2 / PHD1
Host:	Rabbit
Reactivity:	Human, Mouse, Rat, Dog, Guinea pig (tested or 100% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot (0.2 - 1 µg/ml)
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:

EGLN2 / PHD1 antibody was raised against synthetic peptide from C-Terminus of human EGLN2 (Q96KS0, NP_444274). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Mouse, Rat, Guinea pig (100%); Monkey, Marmoset, Elephant (85%); Dog (84%).

Blocking Peptide:

LS-E14429 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110437. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

C-Terminus

Specificity:

Human EGLN2 / PHD1

Usage:

Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Presentation:	Lyophilized from PBS with 2% sucrose
Reconstitution:	Distilled water
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About EGLN2 / PHD1

Uniprot: Q96KS0 NCBI: NM_017555 NP_444274.1

Synonyms: EGLN2 Antibody, EIT6 Antibody, EGL nine (C.elegans) homolog 2 Antibody, Egl nine homolog 2 Antibody, HPH-1 Antibody, HPH-3 Antibody, Estrogen-induced tag 6 Antibody, PHD1 Antibody, HIF-PH1 Antibody, HIF-prolyl hydroxylase 1 Antibody, HIFPH1 Antibody

Description: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A.

(More About EGLN2 / PHD1)

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Western blot

EGLN2 / PHD1 antibody LS-C110437 Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 7/23/2017

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