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Anti-EGLN2 / PHD1 Antibody (aa58-107) IHC-plus™ LS-B1524

Note: This antibody replaces LS-C30009

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular EGLN2 / PHD1 Antibodies

Anti-EGLN2 / PHD1 Antibody (C-Terminus) LS-C49072
Rabbit Polyclonal to Human EGLN2 / PHD1
Human, Mouse
Western blot
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human EGLN2 / PHD1
Human, Bovine, Guinea pig
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human EGLN2 / PHD1
Rabbit
Human, Bovine, Guinea pig (tested or 100% immunogen sequence identity)
Monkey, Mouse, Rat, Dog, Pig (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

EGLN2 / PHD1 antibody was raised against synthetic peptide located between aa58-107 of human EGLN2 (Q96KS0, NP_444274). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%); Galago, Marmoset, Mouse, Rat, Dog, Pig (92%); Elephant, Guinea pig (85%).
LS-E12226 - Lyophilized - 100 µg - $145.00
aa58-107
Human EGLN2 / PHD1
Immunohistochemistry: LS-B1524 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1524 was determined to be 5 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl After adding water, will consist of PBS buffer with 2% sucrose
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About EGLN2 / PHD1

Q96KS0 NM_017555 NP_444274.1

EGLN2 Antibody, EIT6 Antibody, EGL nine (C.elegans) homolog 2 Antibody, Egl nine homolog 2 Antibody, HPH-1 Antibody, HPH-3 Antibody, Estrogen-induced tag 6 Antibody, PHD1 Antibody, HIF-PH1 Antibody, HIF-prolyl hydroxylase 1 Antibody, HIFPH1 Antibody

Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A.

Immunohistochemistry

Immunohistochemistry
Anti-EGLN2 / PHD1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1524 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-EGLN2 / PHD1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1524 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
EGLN2 / PHD1 antibody LS-B1524 Western blot of Placenta lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Placenta Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/7/2016

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