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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EFHC1 Antibody (aa180‑229) LS‑C187196
EFHC1 antibody LS-C187196 is an unconjugated rabbit polyclonal antibody to EFHC1 from human, bovine, guinea pig and other species. Validated for WB.
Catalog
Size
Price
LS-C187196-50
50 µg (1 mg/ml)
Unavailable

Popular EFHC1 Products

Immunohistochemistry of paraffin-embedded rat brain tissue.
Species: Human, Mouse, Rat
Applications: IHC, Western blot
Immunohistochemical analysis of EFHC1 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: Western blot, ELISA
Immunohistochemistry of paraffin-embedded human testis tissue at dilution 1:100
Species: Human
Applications: IHC, ELISA
Immunohistochemistry of paraffin-embedded human testis tissue at dilution 1:100
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

EFHC1 antibody LS-C187196 is an unconjugated rabbit polyclonal antibody to EFHC1 from human, bovine, guinea pig and other species. Validated for WB.
About EFHC1
EFHC1 is an EF-hand-containing calcium binding protein. The encoded protein likely plays a role in calcium homeostasis. Mutations in this gene have been associated with susceptibility to juvenile myoclonic epilepsy and juvenile absence epilepsy. Alternatively spliced transcript variants have been described. Q5JVL4 NM_018100 NP_060570.2

EFHC1 Antibody, DJ304B14.2 Antibody, EJA1 Antibody, EJM1 Antibody, Myoclonin-1 Antibody, JAE Antibody


Specifications

Target
Human EFHC1
Host
Rabbit
Reactivity
Human, Monkey, Bovine, Guinea pig, Pig, Rabbit, Xenopus (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bat, Dog, Hamster, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
EFHC1 antibody was raised against synthetic peptide located between aa180-229 of human EFHC1 (H7C4B1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Elephant, Panda, Bovine, Rabbit, Pig, Opossum, Guinea pig, Platypus, Xenopus (100%); Mouse, Rat, Hamster, Dog, Bat, Horse, Zebra finch (92%); Turkey, Chicken, Sea anemone (84%).
Blocking Peptide
LS-E19436 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C187196. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa180-229
Specificity
Human EFHC1
Presentation
Lyophilized from PBS
Reconstitution
50 µl Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of Human large intestine Tumor. EFHC1 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human large intestine Tumor. EFHC1 antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human large intestine Tumor. EFHC1 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human large intestine Tumor. EFHC1 antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human large intestine Tumor. EFHC1 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human large intestine Tumor. EFHC1 antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human large intestine Tumor. EFHC1 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human large intestine Tumor. EFHC1 antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/17/2018

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