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Anti-EDNRB / Endothelin B Receptor Antibody LS-C149929

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular EDNRB / Endothelin B Receptor Antibodies

Anti-EDNRB / Endothelin B Receptor Antibody (N-Terminus) IHC-plus™ LS-A54
Rabbit Polyclonal to Human EDNRB / Endothelin B Receptor
Human, Dog
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-EDNRB / Endothelin B Receptor Antibody (N-Terminus) IHC-plus™ LS-A55
Rabbit Polyclonal to Human EDNRB / Endothelin B Receptor
Human, Dog
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-EDNRB / Endothelin B Receptor Antibody (Internal, clone EPR7013) IHC-plus™ LS-B8981
Rabbit Monoclonal [clone EPR7013] (IgG) to Human EDNRB / Endothelin B Receptor
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Sheep Polyclonal (IgG) to Human EDNRB / Endothelin B Receptor
Human
ICC, Western blot, Flow Cytometry
Unconjugated

Details

Human EDNRB / Endothelin B Receptor
Sheep
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (5 - 15 µg/ml)
  • Western blot (1 µg/ml)
  • Flow Cytometry

Specificity and Use

EDNRB / Endothelin B Receptor antibody was raised against e. coli-derived recombinant human EDNRB Glu27-Lys 101 Accession # P24530.
Detects human EDNRB in direct ELISAs and Western blots.
Western Blot, 1 µg/ml. Flow Cytometry, 2.5 µg/106 cells. Immunocytochemistry, 5-15 µg/ml.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About EDNRB / Endothelin B Receptor

P24530 NM_000115 NP_000106.1

EDNRB Antibody, ABCDS Antibody, Endothelin B receptor Antibody, Etb receptor Antibody, ET-B Antibody, Et-b receptor Antibody, Et-b-r Antibody, ET-RB Antibody, ETBR Antibody, ETRB Antibody, Etb-svr Antibody, WS4A Antibody, Endothelin receptor type B Antibody, ET-BR Antibody, ETB Antibody, HSCR Antibody, HSCR2 Antibody

The Endothelin Receptor ETB is expressed in most tissues. Mutations of the receptor gene are responsible for Hirschsprung disease type 2, a multigenic disorder with phenotypes such as bicolored irides, megacolon, hypopigmentation, and hearing loss. The ETB receptor has similar affinity for all three endothelins and activates a phosphatidylinositol-calcium second messenger system.

Immunocytochemistry

Immunocytochemistry
EDNRB/Endothelin R Type B in A172 Human Cell Line. EDNRB/Endothelin R Type B was detected in immersion fixed A172 human glioblastoma cell line using 10 ug/ml Sheep Anti-Human EDNRB/Endothelin R Type B Antigen Affinity-purified Polyclonal Antibody for 3 hours at room temperature. Cells were stained with the NorthernLights 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue).

Western blot

Western blot
Detection of Human EDNRB/Endothelin R Type B by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, A1 72 human glioblastoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 ug/ml of Sheep Anti-Human EDNRB/Endothelin R Type B Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for EDNRB/Endothelin R Type B at approximately 38 kD (as indicated). This experiment was conducted under reducing conditions.

Flow Cytometry

Flow Cytometry
Detection of EDNRB/Endothelin R Type B in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Human EDNRB/Endothelin R Type B Antigen Affinity-purified Polyclonal Antibody (filled histogram) or isotype control antibody (open histogram), followed by NorthernLights 557-conjugated Anti-Sheep IgG Secondary Antibody.
 

Requested From: United States
Date Requested: 12/6/2016

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