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Anti-E6AP / UBE3A Antibody (aa836-865) LS-C99579


Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular E6AP / UBE3A Antibodies

Anti-E6AP / UBE3A Antibody (clone 2F6) LS-C133508
Mouse Monoclonal [clone 2F6] (IgG2a,k) to Human E6AP / UBE3A
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human E6AP / UBE3A
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry


Human E6AP / UBE3A
Human (tested or 100% immunogen sequence identity)
Ammonium sulfate precipitation


  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

  • IHC - Paraffin

Specificity and Use

E6AP / UBE3A antibody was raised against kLH-conjugated synthetic peptide between amino acids 836-865 from C-terminal region of human UBE3A.
LS-E6902 - Lyophilized - 100 µg - $145.00


PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About E6AP / UBE3A

Q05086 NM_000462 NP_000453.2

UBE3A Antibody, ANCR Antibody, E6-ap isoform-III Antibody, E6AP ubiquitin-protein ligase Antibody, EPVE6AP Antibody, HPVE6A Antibody, AS Antibody, CTCL tumor antigen se37-2 Antibody, E6-AP Antibody, E6AP Antibody, Ubiquitin protein ligase E3A Antibody, Ubiquitin-protein ligase E3A Antibody

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B.


Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.


Confocal immunofluorescence of UBE3A Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot
Western blot of UBE3A Antibody in T47D cell line lysates (35 ug/lane). UBE3A(arrow) was detected using the purified antibody.

Flow Cytometry

Flow Cytometry
UBE3A Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 10/26/2016

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