Reactivity
Drosophila
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-TKGLFQTPYRSARN, from the internal region of the protein sequence according to NP_524437.2.
Specificity
Drosophila E2F. Reported isoforms represent identical protein: NP_524437.2; NP_732646.1; NP_732647.1
Applications
- Immunofluorescence (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Immunofluorescence: This antibody shows identical pattern in the Drosophila eye-antennal imaginal disc as was observed before with a different antibody (Moon et al, Mol Cell Biol. 2006 Oct;26(20):7601-15. PMID: 16954388). Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee