Bovine DSG1+2 / Desmoglein 1+2
Bovine, Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Protein A purified
- IHC - Paraffin
- IHC - Frozen
- Western blot
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Band 3 polypeptide of isolated desmosomes from bovine muzzle epidermis.
Species (tested so far) Human, bovine, rat, mouse. Recognizes desmoglein 1 + 2 (desmosome-specific cadherins) in desmosome-bearing cells; does not react with cells containing plaque-bearing junctions of non-desmosomal type. Tumors specifically detected: all epithelium-derived tumors, notably carcinomas, and meningiomas. Polypeptide reacting: Mr 165000 Desmoglein, 'band 3' polypeptide of the desmosomal complex. Epitope recognized has been localized at the repeating unit domain ('RUD') of the cytoplasmic C-terminal part of the polypeptide. Reactivities on Cultured Cell Lines (tested so far): A-431, MCF-7, TR-146, PLC and various other human epithelial cell lines; bovine BMGE+H, BMGE-H, MDBK.
Suitable for use in Immunohistochemistry (frozen sections, paraffin-embedded tissue after microwave treatment), Western Blot; Cryo, Paraffin (Microwave), Immunofluorescence microscopy. Immunohistochemistry: 1:10 with PBS, pH 7.4; Incubation Time 1 h at RT; extended with paraffin.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.