(tested or 100% immunogen sequence identity)
DPPA4 antibody was raised against e. coli-derived rmDPPA4 (aa125-296).
Selected for its ability to recognize mouse DPPA4 in the applications listed. In direct ELISAs and Western Blots, this antibody shows less than 5% cross-reactivity with rhDPPA4 and rmDPPA5.
Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect mouse DPPA4. The detection limit for rmDPPA4 is approximately 1 ng/lane under non-reducing and reducing conditions. This antibody has not been optimized for Western blot detection of DPPA4 in cell lysates. Immunocytochemistry: This antibody has been used at a concentration of 10 ug/ml to detect mouse DPPA4 in mouse embryonic stem cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect mouse DPPA4. The detection limit for rmDPPA4 is approximately 0.2 ng/well.
100 µl deionized water
For research use only.
This antibody carries the LSBio 100% Guarantee.