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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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DPPA4 Antibody (aa125‑296) LS‑C36162 Best Seller
DPPA4 antibody LS-C36162 is an unconjugated goat polyclonal antibody to mouse DPPA4. Validated for ELISA, ICC and WB.
Antibody
Catalog
Size
Price
LS-C36162-100
100 µg
Unavailable

Popular DPPA4 Products

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Product Description

DPPA4 antibody LS-C36162 is an unconjugated goat polyclonal antibody to mouse DPPA4. Validated for ELISA, ICC and WB.
About DPPA4
May be involved in the maintenance of active epigenetic status of target genes. May inhibit differentiation of embryonic cells into a primitive ectoderm lineage. Q7L190 NM_018189 NP_060659.3

DPPA4 Antibody


Specifications

Target
Mouse DPPA4
Host
Goat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • ICC
  • Western blot
  • ELISA
Immunogen
DPPA4 antibody was raised against e. coli-derived rmDPPA4 (aa125-296).
Epitope
aa125-296
Specificity
Selected for its ability to recognize mouse DPPA4 in the applications listed. In direct ELISAs and Western Blots, this antibody shows less than 5% cross-reactivity with rhDPPA4 and rmDPPA5.
Usage
Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect mouse DPPA4. The detection limit for rmDPPA4 is approximately 1 ng/lane under non-reducing and reducing conditions. This antibody has not been optimized for Western blot detection of DPPA4 in cell lysates. Immunocytochemistry: This antibody has been used at a concentration of 10 ug/ml to detect mouse DPPA4 in mouse embryonic stem cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect mouse DPPA4. The detection limit for rmDPPA4 is approximately 0.2 ng/well.
Presentation
Lyophilized.
Reconstitution
100 µl deionized water
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 7/19/2018

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