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DOK1 Antibody (C‑Terminus) IHC‑plus™ LS‑B561
Note: This antibody replaces LS-C253, LS-C123405
DOK1 antibody LS-B561 is an unconjugated rabbit polyclonal antibody to human DOK1. Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
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Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse, Rat, Bovine
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, Immunofluorescence, Western blot, ELISA
Product Description
DOK1 antibody LS-B561 is an unconjugated rabbit polyclonal antibody to human DOK1. Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About DOK1
DOK1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. DOK1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. Docking protein 2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.
Q99704
NM_001381
NP_001372.1
DOK1 Antibody, Docking protein 1 Antibody, Gap binding protein p62dok Antibody, p62(dok) Antibody, p62DOK Antibody, Pp62 Antibody
Specifications
Target
Human DOK1
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- ICC
- Western blot (0.5 - 1 µg/ml)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Blocking Peptide
LS-E29835 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B561. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunizing peptide used to generate LS-B561. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Usage
Immunohistochemistry: LS-B561 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B561 was determined to be 5 ug/ml.
Presentation
PBS, 0.02% sodium azide.
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images
Immunohistochemistry
Anti-DOK1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.
Immunohistochemistry
Anti-DOK1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.
Immunocytochemistry
Immunocytochemistry of DOK1 in K562 cells with DOK1 antibody at 2 ug/ml.
Immunofluorescence
Immunofluorescence of DOK1 in K562 cells with DOK1 antibody at 10 ug/ml.
Western blot
Western blot of DOK1 in Jurkat (Jur) and THP-1 (THP) cell lysates with DOK1 antibody at 1 ug/ml.
Immunohistochemistry
Anti-DOK1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.
Immunohistochemistry
Anti-DOK1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.
Immunocytochemistry
Immunocytochemistry of DOK1 in K562 cells with DOK1 antibody at 2 ug/ml.
Immunofluorescence
Immunofluorescence of DOK1 in K562 cells with DOK1 antibody at 10 ug/ml.
Western blot
Western blot of DOK1 in Jurkat (Jur) and THP-1 (THP) cell lysates with DOK1 antibody at 1 ug/ml.
Immunohistochemistry
Anti-DOK1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.
Immunohistochemistry
Anti-DOK1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.
Immunocytochemistry
Immunocytochemistry of DOK1 in K562 cells with DOK1 antibody at 2 ug/ml.
Immunofluorescence
Immunofluorescence of DOK1 in K562 cells with DOK1 antibody at 10 ug/ml.
Western blot
Western blot of DOK1 in Jurkat (Jur) and THP-1 (THP) cell lysates with DOK1 antibody at 1 ug/ml.
Immunohistochemistry
Anti-DOK1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.
Immunohistochemistry
Anti-DOK1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.
Immunocytochemistry
Immunocytochemistry of DOK1 in K562 cells with DOK1 antibody at 2 ug/ml.
Immunofluorescence
Immunofluorescence of DOK1 in K562 cells with DOK1 antibody at 10 ug/ml.
Western blot
Western blot of DOK1 in Jurkat (Jur) and THP-1 (THP) cell lysates with DOK1 antibody at 1 ug/ml.
Requested From:
Date Requested: 6/19/2018
Date Requested: 6/19/2018
