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Anti-DOK1 Antibody (C-Terminus) IHC-plus™ LS-B561
Note: This antibody replaces LS-C253, LS-C123405Most Popular DOK1 Antibodies
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Details
| Human DOK1 | |
| Rabbit | |
| Human (tested or 100% immunogen sequence identity) | |
| Polyclonal | |
| Unconjugated | |
| Immunoaffinity purified | |
| Unmodified |
Applications
- IHC - Paraffin (5 µg/ml)
- ICC
- Western blot (0.5 - 1 µg/ml)
Specificity and Use
| DOK1 antibody was raised against synthetic peptide from human DOK1. | ||
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| C-Terminus | ||
| peptide corresponding to amino acids near the carboxy terminus of human p62dok | ||
| Immunohistochemistry: LS-B561 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B561 was determined to be 5 ug/ml. |
Packaging
| PBS, 0.02% sodium azide. | |
| Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted. | |
| For research use only. |
About DOK1
Q99704
NM_001381
NP_001372.1
DOK1 Antibody, Docking protein 1 Antibody, Gap binding protein p62dok Antibody, p62(dok) Antibody, p62DOK Antibody, Pp62 Antibody
DOK1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. DOK1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. Docking protein 2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.
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Requested From:
Date Requested: 8/19/2017
Date Requested: 8/19/2017
