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Anti-DOK1 Antibody (C-Terminus) IHC-plus™ LS-B561

Note: This antibody replaces LS-C253, LS-C123405
Catalog Size Price
LS-B561-50 50 µg (0.5 mg/ml) Unavailable

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59 DOK1 Antibodies

Most Popular DOK1 Antibodies

Anti-DOK1 Antibody (C-Terminus) IHC-plus™ LS-B561
Rabbit Polyclonal to Human DOK1
Human
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-DOK1 Antibody (Tyr398) LS-C34463
Rabbit Polyclonal (IgG) to Human DOK1
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot
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Immunohistochemistry Image
Anti-DOK1 Antibody (Internal) LS-C368740
Rabbit Polyclonal to Human DOK1
Human, Mouse, Rat, Bovine
IHC - Paraffin, ICC, Immunofluorescence, Western blot
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Immunohistochemistry Image
Anti-DOK1 Antibody (aa300-380) LS-C383183
Rabbit Polyclonal (IgG) to Human DOK1
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot, ELISA
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Anti-DOK1 Antibody (aa340-420) LS-C383185
Rabbit Polyclonal (IgG) to Human DOK1
Human, Mouse, Rat
IHC, Western blot, ELISA
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100% Guaranteed
Rabbit Polyclonal to Human DOK1
Human
IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human DOK1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Western blot (0.5 - 1 µg/ml)

Specificity and Use

DOK1 antibody was raised against synthetic peptide from human DOK1.
LS-E29835 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B561. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
C-Terminus
peptide corresponding to amino acids near the carboxy terminus of human p62dok
Immunohistochemistry: LS-B561 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B561 was determined to be 5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About DOK1

Q99704 NM_001381 NP_001372.1

DOK1 Antibody, Docking protein 1 Antibody, Gap binding protein p62dok Antibody, p62(dok) Antibody, p62DOK Antibody, Pp62 Antibody

DOK1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. DOK1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. Docking protein 2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.

Immunohistochemistry

Immunohistochemistry
Anti-DOK1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-DOK1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry
Immunocytochemistry of DOK1 in K562 cells with DOK1 antibody at 2 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of DOK1 in K562 cells with DOK1 antibody at 10 ug/ml.

Western blot

Western blot
Western blot of DOK1 in Jurkat (Jur) and THP-1 (THP) cell lysates with DOK1 antibody at 1 ug/ml.

Requested From: 
Date Requested: 10/23/2017

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