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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DOK1 Antibody (C‑Terminus) IHC‑plus™ LS‑B561 Best Seller
Note: This antibody replaces LS-C253, LS-C123405
DOK1 antibody LS-B561 is an unconjugated rabbit polyclonal antibody to human DOK1. Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B561-50
50 µg (0.5 mg/ml)
Unavailable

Popular DOK1 Products

Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using p62Dok(Ab-398) antibody (left) or the same antibody preincubated with blocking peptide (right).
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
IHC of paraffin-embedded human breast carcinoma tissue, using p62 Dok (Ab-362) Antibody. The picture on the right is treated with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemical analysis of DOK1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Bovine
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Western blot of Dok-1 antibody
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

DOK1 antibody LS-B561 is an unconjugated rabbit polyclonal antibody to human DOK1. Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About DOK1
DOK1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. DOK1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. Docking protein 2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling. Q99704 NM_001381 NP_001372.1

DOK1 Antibody, Docking protein 1 Antibody, Gap binding protein p62dok Antibody, p62(dok) Antibody, p62DOK Antibody, Pp62 Antibody


Specifications

Target
Human DOK1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Western blot (0.5 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E29835 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B561. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Usage
Immunohistochemistry: LS-B561 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B561 was determined to be 5 ug/ml.
Presentation
PBS, 0.02% sodium azide.
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-DOK1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.
Anti-DOK1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.

Immunohistochemistry

Anti-DOK1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.
Anti-DOK1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of DOK1 in K562 cells with DOK1 antibody at 2 ug/ml.
Immunocytochemistry of DOK1 in K562 cells with DOK1 antibody at 2 ug/ml.

Immunofluorescence

Immunofluorescence of DOK1 in K562 cells with DOK1 antibody at 10 ug/ml.
Immunofluorescence of DOK1 in K562 cells with DOK1 antibody at 10 ug/ml.

Western blot

Western blot of DOK1 in Jurkat (Jur) and THP-1 (THP) cell lysates with DOK1 antibody at 1 ug/ml.
Western blot of DOK1 in Jurkat (Jur) and THP-1 (THP) cell lysates with DOK1 antibody at 1 ug/ml.

Immunohistochemistry

Anti-DOK1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.
Anti-DOK1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.

Immunohistochemistry

Anti-DOK1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.
Anti-DOK1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of DOK1 in K562 cells with DOK1 antibody at 2 ug/ml.
Immunocytochemistry of DOK1 in K562 cells with DOK1 antibody at 2 ug/ml.

Immunofluorescence

Immunofluorescence of DOK1 in K562 cells with DOK1 antibody at 10 ug/ml.
Immunofluorescence of DOK1 in K562 cells with DOK1 antibody at 10 ug/ml.

Western blot

Western blot of DOK1 in Jurkat (Jur) and THP-1 (THP) cell lysates with DOK1 antibody at 1 ug/ml.
Western blot of DOK1 in Jurkat (Jur) and THP-1 (THP) cell lysates with DOK1 antibody at 1 ug/ml.

Immunohistochemistry

Anti-DOK1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.
Anti-DOK1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.

Immunohistochemistry

Anti-DOK1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.
Anti-DOK1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of DOK1 in K562 cells with DOK1 antibody at 2 ug/ml.
Immunocytochemistry of DOK1 in K562 cells with DOK1 antibody at 2 ug/ml.

Immunofluorescence

Immunofluorescence of DOK1 in K562 cells with DOK1 antibody at 10 ug/ml.
Immunofluorescence of DOK1 in K562 cells with DOK1 antibody at 10 ug/ml.

Western blot

Western blot of DOK1 in Jurkat (Jur) and THP-1 (THP) cell lysates with DOK1 antibody at 1 ug/ml.
Western blot of DOK1 in Jurkat (Jur) and THP-1 (THP) cell lysates with DOK1 antibody at 1 ug/ml.

Immunohistochemistry

Anti-DOK1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.
Anti-DOK1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.

Immunohistochemistry

Anti-DOK1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.
Anti-DOK1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B561 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of DOK1 in K562 cells with DOK1 antibody at 2 ug/ml.
Immunocytochemistry of DOK1 in K562 cells with DOK1 antibody at 2 ug/ml.

Immunofluorescence

Immunofluorescence of DOK1 in K562 cells with DOK1 antibody at 10 ug/ml.
Immunofluorescence of DOK1 in K562 cells with DOK1 antibody at 10 ug/ml.

Western blot

Western blot of DOK1 in Jurkat (Jur) and THP-1 (THP) cell lysates with DOK1 antibody at 1 ug/ml.
Western blot of DOK1 in Jurkat (Jur) and THP-1 (THP) cell lysates with DOK1 antibody at 1 ug/ml.


Requested From: 
Date Requested: 6/19/2018

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