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Anti-DLX5 Antibody (aa107-120) IHC-plus™ LS-B6489

Note: This antibody replaces LS-C112830
Catalog Size Price
LS-B6489-50 50 µg (0.5 mg/ml) Unavailable

Related Products

50 DLX5 Antibodies

Most Popular DLX5 Antibodies

Anti-DLX5 Antibody (aa29-45) LS-C6400
Rabbit Polyclonal (IgG) to Human DLX5
Human, Monkey, Mouse, Rat, Bat, Bovine, Hamster, Horse, Pig, Chicken
IHC, IHC - Frozen, Western blot
Unconjugated
Anti-DLX5 Antibody (aa120-169) LS-C80030
Rabbit Polyclonal (IgG) to Human DLX5
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
IHC, IHC - Paraffin, Western blot
Unconjugated
DLX5 antibody LS-C80030 was used in IHC to stain formalin-fixed, paraffin-embedded human lung.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-DLX5 Antibody (aa107-120) IHC-plus™ LS-B6489
Goat Polyclonal to Human DLX5
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Anti-DLX5 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6489 concentration 2.5 ug/ml.
Anti-DLX5 Antibody (Internal) LS-C352118
Rabbit Polyclonal to Human DLX5
Human, Mouse, Rat, Chicken, Zebrafish
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemical analysis of DLX5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Anti-DLX5 Antibody (aa60-140) LS-C383155
Rabbit Polyclonal (IgG) to Human DLX5
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Western blot of Dlx-5 antibody

100% Guaranteed
Goat Polyclonal to Human DLX5
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human DLX5
Goat
Human (tested or 100% immunogen sequence identity)
Monkey, Mouse, Rat, Bovine, Horse, Pig (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (2.5 - 3.75 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • ELISA (1:32000)

Specificity and Use

DLX5 antibody was raised against synthetic peptide C-AYNRVPSATNQPEK from an internal region of human DLX5 (NP_005212.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%); Monkey, Mouse, Rat, Panda, Bovine, Horse, Pig (93%); Hamster, Elephant, Dog, Bat (86%).
LS-E27032 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B6489. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa107-120
Human DLX5.
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 30kDa band observed in Human Bone Marrow lysates (calculated MW of 31.5kDa according to NP_005212.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lee et al, J Biol Chem. 2003 Sep 5;278(36):34387-94; PMID: 12815054). Recommended concentration: 1-3 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About DLX5

P56178 NM_005221 NP_005212.1

DLX5 Antibody, Distal-less homeobox 5 Antibody, Homeobox protein DLX-5 Antibody, Distal-less homeo box 5 Antibody, SHFM1D Antibody

Transcriptional factor involved in bone development. Acts as an immediate early BMP-responsive transcriptional activator essential for osteoblast differentiation. Stimulates ALPL promoter activity in a RUNX2-independent manner during osteoblast differentiation. Stimulates SP7 promoter activity during osteoblast differentiation. Promotes cell proliferation by up-regulating MYC promoter activity.

Immunohistochemistry

Anti-DLX5 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6489 concentration 2.5 ug/ml.
Anti-DLX5 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6489 concentration 2.5 ug/ml.

Western blot

DLX5 antibody (1 ug/ml) staining of Human Bone Marrow lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
DLX5 antibody (1 ug/ml) staining of Human Bone Marrow lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 4/25/2018

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