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Antibodies > Digoxin > LS-C355944
 
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Catalog Number Size Price
LS-C355944-1 1 mg $734 
Digoxin Antibody - Western blot analysis of Digoxin was performed by loading 10ng of BSA or Digoxin-labeled BSA per well and 5ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Blotter, and blocked with 5% milk in TBST for 1 hour at room temperature. BSA was detected at ~66kD using a BSA monoclonal antibody at a dilution of 1 µg/mL in 5% milk in TBST overnight at 4C on a rocking platform. Digoxin-labeled BSA was detected at ~66kD using a Digoxin monoclonal antibody at a dilution of 1 µg/mL in 5% milk in TBST overnight at 4C on a rocking platform. After washing, blots were probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico substrate.
Digoxin Antibody - Chemiluminescent detection of a Digoxin-labeled oligonucleotide was performed by diluting a Digoxin-labeled DNA oligonucleotide (+) or an unlabeled oligonucleotide control (-) in TriTracker Loading Dye, and loading 5pmols onto a 6% DNA Retardation Gel. DNA was transferred to a Biodyne B membrane and blocked for 30 minutes at room temperature. Digoxin was detected using a Digoxin monoclonal antibody at a dilution of 1 µg/mL for 30 minutes at room temperature, followed by a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature. Detection was performed using the Chemiluminescent Nucleic Acid Detection Module Kit.
Digoxin Antibody - Indirect ELISA analysis of Digoxin was performed by preparing a 10 µg/mL solution of BSA or Digoxin-labeled BSA in 0.2M Carbonate-Bicarbonate, pH 9.4. 100uL of each solution was added to separate wells of a clear 96-well plate, and incubated overnight at 4C. Each well was blocked with SuperBlock in PBS. The plate was then washed with PBST and incubated with 100uL per well of Digoxin monoclonal antibody in triplicate at ~1000 - 2pg/mL for 1 hour at room temperature. The plate was washed with PBST and incubated with 100ul per well of goat anti-mouse IgG-HRP secondary antibody in all test wells at 1:25,000 for 30 minutes at room temperature. Detection was performed using 1-Step TMB Ultra substrate for 15 minutes at room temperature. The plate was then stopped with 0.2M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.
Digoxin Antibody - Western blot analysis of Digoxin was performed by loading 10ng of BSA or Digoxin-labeled BSA per well and 5ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Blotter, and blocked with 5% milk in TBST for 1 hour at room temperature. BSA was detected at ~66kD using a BSA monoclonal antibody at a dilution of 1 µg/mL in 5% milk in TBST overnight at 4C on a rocking platform. Digoxin-labeled BSA was detected at ~66kD using a Digoxin monoclonal antibody at a dilution of 1 µg/mL in 5% milk in TBST overnight at 4C on a rocking platform. After washing, blots were probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico substrate.
Digoxin Antibody - Chemiluminescent detection of a Digoxin-labeled oligonucleotide was performed by diluting a Digoxin-labeled DNA oligonucleotide (+) or an unlabeled oligonucleotide control (-) in TriTracker Loading Dye, and loading 5pmols onto a 6% DNA Retardation Gel. DNA was transferred to a Biodyne B membrane and blocked for 30 minutes at room temperature. Digoxin was detected using a Digoxin monoclonal antibody at a dilution of 1 µg/mL for 30 minutes at room temperature, followed by a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature. Detection was performed using the Chemiluminescent Nucleic Acid Detection Module Kit.
Digoxin Antibody - Indirect ELISA analysis of Digoxin was performed by preparing a 10 µg/mL solution of BSA or Digoxin-labeled BSA in 0.2M Carbonate-Bicarbonate, pH 9.4. 100uL of each solution was added to separate wells of a clear 96-well plate, and incubated overnight at 4C. Each well was blocked with SuperBlock in PBS. The plate was then washed with PBST and incubated with 100uL per well of Digoxin monoclonal antibody in triplicate at ~1000 - 2pg/mL for 1 hour at room temperature. The plate was washed with PBST and incubated with 100ul per well of goat anti-mouse IgG-HRP secondary antibody in all test wells at 1:25,000 for 30 minutes at room temperature. Detection was performed using 1-Step TMB Ultra substrate for 15 minutes at room temperature. The plate was then stopped with 0.2M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.
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Monoclonal Mouse Digoxin Antibody (clone 26G10, WB) LS‑C355944

Monoclonal Mouse Digoxin Antibody (clone 26G10, WB) LS‑C355944

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Antibody:
Digoxin Mouse Monoclonal (26G10) Antibody
Application:
WB, IP, ELISA, RIA
Format:
Unconjugated, Unmodified
Price
Catalog Number
$734
LS-C355944-1
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
Digoxin Mouse Monoclonal (26G10) Antibody
Application:
WB, IP, ELISA, RIA
Format:
Unconjugated, Unmodified

Specifications

Description
Digoxin antibody LS-C355944 is an unconjugated mouse monoclonal antibody to Digoxin. Validated for ELISA, IP, RIA and WB.
Target
Digoxin
Host
Mouse
Clonality
IgG1 Monoclonal
Clone
26G10
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Immunogen
Digoxin-BSA.
Specificity
The antibody targets Digoxin in ELISA, IP, and RIA applications and shows reactivity with Chemical samples.
Applications
  • Western blot
  • Immunoprecipitation
  • ELISA
  • Radioimmunoassay
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/26/2024
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