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DIAPH1 Antibody (aa1196‑1245) LS‑C151574
DIAPH1 antibody LS-C151574 is an unconjugated rabbit polyclonal antibody to DIAPH1 from human, bat, bovine and other species. Validated for WB.
Catalog
Size
Price
LS-C151574-50
50 µg (1 mg/ml)
Unavailable

Popular DIAPH1 Products

DIAPH1 antibody (0.3 ug/ml) staining of NIH3T3 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Species: Human, Mouse, Rat, Bat
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
IHC of paraffin-embedded gastric tissue using DIAPH1 antibody at 1:100 dilution.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot
Detection of Human Diaphanous by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Diaphanous used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Pig
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation

Product Description

DIAPH1 antibody LS-C151574 is an unconjugated rabbit polyclonal antibody to DIAPH1 from human, bat, bovine and other species. Validated for WB.
About DIAPH1
Acts in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. O60610 NM_005219 NP_005210.3

DIAPH1 Antibody, DRF1 Antibody, DIA1 Antibody, Diaphanous-related formin 1 Antibody, Diaphanous-related formin-1 Antibody, DIAP1 Antibody, Protein diaphanous homolog 1 Antibody, DFNA1 Antibody, HDIA1 Antibody, LFHL1 Antibody


Specifications

Target
Human DIAPH1
Host
Rabbit
Reactivity
Human, Monkey, Bat, Bovine, Dog, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Pig (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
DIAPH1 antibody was raised against synthetic peptide located between aa1196-1245 of human DIAPH1 (O60610, NP_005210). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Galago, Marmoset, Elephant, Dog, Bat, Opossum (100%); Gibbon, Mouse, Rat, Lizard (92%); Pig (91%); Rabbit (85%); Bovine (84%).
Blocking Peptide
LS-E23306 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C151574. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1196-1245
Specificity
Human DIAPH1
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

DIAPH1 antibody Western Blot of HepG2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DIAPH1 antibody Western Blot of HepG2. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

DIAPH1 antibody Western Blot of HepG2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DIAPH1 antibody Western Blot of HepG2. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

DIAPH1 antibody Western Blot of HepG2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DIAPH1 antibody Western Blot of HepG2. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

DIAPH1 antibody Western Blot of HepG2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DIAPH1 antibody Western Blot of HepG2. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/22/2018

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