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Anti-DIABLO / SMAC Antibody (aa56-239) LS-C19112

Ordering

Wt. Vol. Conc. Price
- 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular DIABLO / SMAC Antibodies

Anti-DIABLO / SMAC Antibody (aa225-239) LS-C4
Rabbit Polyclonal to Human DIABLO / SMAC
Human, Mouse, Rat
IHC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-DIABLO / SMAC Antibody (aa222-237) IHC-plus™ LS-B1350
Rabbit Polyclonal to Mouse DIABLO / SMAC
Mouse, Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-DIABLO / SMAC Antibody (aa226-239) IHC-plus™ LS-B6501
Rabbit Polyclonal (IgG) to Human DIABLO / SMAC
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human DIABLO / SMAC
Human
Western blot
Unconjugated

Details

Human DIABLO / SMAC
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Unmodified

Applications

Western blot

Specificity and Use

DIABLO / SMAC antibody was raised against recombinant human DIABLO / SMAC.
aa56-239
Recombinant His6-tagged human Smac/DIABLO protein (amino acids 56-239).
This antibody reacts with human Smac/DIABLO protein by immunoblot. Lysates from human HeLa and LNCaP cells are positive for Smac/DIABLO. Other animal tissues have not been tested.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
For research use only.

About DIABLO / SMAC

Q9NR28 NM_019887 NP_063940.1

DIABLO Antibody, DFNA64 Antibody, DIABLO-S Antibody, Diablo homolog, mitochondrial Antibody, SMAC3 Antibody, Mitochondrial Smac protein Antibody, SMAC Antibody

Second mitochondria-derived activator of caspase (SMAC) has been implicated in the activation of apoptosis in response to cell stress. SMAC promotes CASP9 activation by binding to IAPs and removing their inhibitory activity. The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes.

Western blot

Western blot
Western blot of anti-Smac detects a 26 kDa band when 1 ug of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 M etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1

Western blot

Western blot
Anti-Smac is shown to detect a 25-26 kDa band in partially purified recombinant human Smac protein by western blot. Lanes 1-3 are loaded with 1, 10 and 100 ng of protein per lane, respectively. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1. Other detection systems will yield similar results.

Western blot

Western blot
Western blot of anti-Smac detects a 26 kD band when 1 ug of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 uM etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. Antibody was used at a 1:1000 dilution.
 

Requested From: United States
Date Requested: 12/7/2016

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