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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DIABLO / SMAC Antibody (aa56‑239) LS‑C19112
SMAC antibody LS-C19112 is an unconjugated rabbit polyclonal antibody to human SMAC (DIABLO). Validated for WB.
Catalog
Size
Price
LS-C19112-100
100 µl
Unavailable

Popular DIABLO / SMAC Products

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Anti-DIABLO / SMAC antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B221 dilution 1:500.
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Anti-DIABLO / SMAC antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1350 concentration 5 ug/ml.
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Anti-DIABLO / SMAC antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3668 concentration 10 ug/ml.
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Product Description

SMAC antibody LS-C19112 is an unconjugated rabbit polyclonal antibody to human SMAC (DIABLO). Validated for WB.
About DIABLO / SMAC
Second mitochondria-derived activator of caspase (SMAC) has been implicated in the activation of apoptosis in response to cell stress. SMAC promotes CASP9 activation by binding to IAPs and removing their inhibitory activity. The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. Q9NR28 NM_019887 NP_063940.1

DIABLO Antibody, DFNA64 Antibody, DIABLO-S Antibody, Diablo homolog, mitochondrial Antibody, SMAC3 Antibody, Mitochondrial Smac protein Antibody, SMAC Antibody


Specifications

Target
Human DIABLO / SMAC
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Applications
  • Western blot
Immunogen
DIABLO / SMAC antibody was raised against recombinant human DIABLO / SMAC.
Epitope
aa56-239
Specificity
Recombinant His6-tagged human Smac/DIABLO protein (amino acids 56-239).
Usage
This antibody reacts with human Smac/DIABLO protein by immunoblot. Lysates from human HeLa and LNCaP cells are positive for Smac/DIABLO. Other animal tissues have not been tested.
Presentation
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Storage
Store vial at -20°C. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Dilute only prior to immediate use.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of anti-Smac detects a 26 kDa band when 1 ug of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 M etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1
Western blot of anti-Smac detects a 26 kDa band when 1 ug of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 M etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1

Western blot

Anti-Smac is shown to detect a 25-26 kDa band in partially purified recombinant human Smac protein by western blot. Lanes 1-3 are loaded with 1, 10 and 100 ng of protein per lane, respectively. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1. Other detection systems will yield similar results.
Anti-Smac is shown to detect a 25-26 kDa band in partially purified recombinant human Smac protein by western blot. Lanes 1-3 are loaded with 1, 10 and 100 ng of protein per lane, respectively. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1. Other detection systems will yield similar results.

Western blot

Western blot of anti-Smac detects a 26 kD band when 1 ug of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 uM etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. Antibody was used at a 1:1000 dilution.
Western blot of anti-Smac detects a 26 kD band when 1 ug of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 uM etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. Antibody was used at a 1:1000 dilution.

Western blot

Western blot of anti-Smac detects a 26 kDa band when 1 ug of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 M etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1
Western blot of anti-Smac detects a 26 kDa band when 1 ug of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 M etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1

Western blot

Anti-Smac is shown to detect a 25-26 kDa band in partially purified recombinant human Smac protein by western blot. Lanes 1-3 are loaded with 1, 10 and 100 ng of protein per lane, respectively. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1. Other detection systems will yield similar results.
Anti-Smac is shown to detect a 25-26 kDa band in partially purified recombinant human Smac protein by western blot. Lanes 1-3 are loaded with 1, 10 and 100 ng of protein per lane, respectively. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1. Other detection systems will yield similar results.

Western blot

Western blot of anti-Smac detects a 26 kD band when 1 ug of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 uM etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. Antibody was used at a 1:1000 dilution.
Western blot of anti-Smac detects a 26 kD band when 1 ug of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 uM etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. Antibody was used at a 1:1000 dilution.

Western blot

Western blot of anti-Smac detects a 26 kDa band when 1 ug of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 M etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1
Western blot of anti-Smac detects a 26 kDa band when 1 ug of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 M etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1

Western blot

Anti-Smac is shown to detect a 25-26 kDa band in partially purified recombinant human Smac protein by western blot. Lanes 1-3 are loaded with 1, 10 and 100 ng of protein per lane, respectively. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1. Other detection systems will yield similar results.
Anti-Smac is shown to detect a 25-26 kDa band in partially purified recombinant human Smac protein by western blot. Lanes 1-3 are loaded with 1, 10 and 100 ng of protein per lane, respectively. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1. Other detection systems will yield similar results.

Western blot

Western blot of anti-Smac detects a 26 kD band when 1 ug of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 uM etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. Antibody was used at a 1:1000 dilution.
Western blot of anti-Smac detects a 26 kD band when 1 ug of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 uM etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. Antibody was used at a 1:1000 dilution.

Western blot

Western blot of anti-Smac detects a 26 kDa band when 1 ug of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 M etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1
Western blot of anti-Smac detects a 26 kDa band when 1 ug of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 M etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1

Western blot

Anti-Smac is shown to detect a 25-26 kDa band in partially purified recombinant human Smac protein by western blot. Lanes 1-3 are loaded with 1, 10 and 100 ng of protein per lane, respectively. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1. Other detection systems will yield similar results.
Anti-Smac is shown to detect a 25-26 kDa band in partially purified recombinant human Smac protein by western blot. Lanes 1-3 are loaded with 1, 10 and 100 ng of protein per lane, respectively. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1. Other detection systems will yield similar results.

Western blot

Western blot of anti-Smac detects a 26 kD band when 1 ug of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 uM etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. Antibody was used at a 1:1000 dilution.
Western blot of anti-Smac detects a 26 kD band when 1 ug of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 uM etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. Antibody was used at a 1:1000 dilution.


Requested From: 
Date Requested: 6/20/2018

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