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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DHX15 Antibody (aa686‑735) LS‑C80156 Best Seller
DHX15 antibody LS-C80156 is an unconjugated rabbit polyclonal antibody to DHX15 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C80156-50
50 µg
Unavailable

Popular DHX15 Products

Detection of Human DHX15 by Immunohistochemistry. Sample: FFPE section of human ovarian tumor. Antibody: Affinity purified rabbit anti-DHX15 used at a dilution of 1:250.
Species: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Rabbit
Applications: IHC, IHC - Paraffin, Immunofluorescence
Detection of Human DHX15 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DHX15 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
Species: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Rabbit
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Anti-DHX15 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11210 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

DHX15 antibody LS-C80156 is an unconjugated rabbit polyclonal antibody to DHX15 from human, mouse, rat and other species. Validated for WB.
About DHX15
DHX15 is a pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns. O43143 NM_001358 NP_001349.2

DHX15 Antibody, ATP-dependent RNA helicase #46 Antibody, DEAH box protein 15 Antibody, DDX15 Antibody, DEAD/H box-15 Antibody, PRP43 Antibody, PRPF43 Antibody, RNA helicase 2 Antibody, DBP1 Antibody, PrPp43p Antibody


Specifications

Target
Human DHX15
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, HRP, Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
DHX15 antibody was raised against synthetic peptide located between aa686-735 of human DHX15 (O43143, NP_001349). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Lizard, Xenopus, Zebrafish, Sea squirt (100%); Gibbon, Grape, Arabidopsis (92%); Drosophila, Beetle (85%); Nematode (83%).
Blocking Peptide
LS-E13011 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80156. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa686-735
Specificity
Human DHX15
Usage
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Western blot

293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 7/15/2018

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