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Anti-DHAND / HAND2 Antibody (aa81-110) LS-C166783

Catalog Size Price
LS-C166783-400 400 µl Unavailable

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100% Guaranteed
Rabbit Polyclonal to Human DHAND / HAND2
Human
Immunofluorescence, Western blot
Unconjugated

Details

Human DHAND / HAND2
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse, Rat, Chicken, Xenopus (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)

Specificity and Use

LS-E4646 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C166783. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa81-110
This HAND2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-110 amino acids from the Central region of human HAND2.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About DHAND / HAND2

P61296 NM_021973 NP_068808.1

HAND2 Antibody, DHand Antibody, DHAND2 Antibody, Hed Antibody, Thing2 Antibody, BHLHa26 Antibody

Essential for cardiac morphogenesis, particularly for the formation of the right ventricle and of the aortic arch arteries. Required for vascular development and regulation of angiogenesis, possibly through a VEGF signaling pathway. Plays also an important role in limb development, particularly in the establishment of anterior-posterior polarization, acting as an upstream regulator of sonic hedgehog (SHH) induction in the limb bud.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of HeLa cell stained with HAND2 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HAND2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HAND2 immunoreactivity is localized to Nucleus significantly.

Western blot

Western blot
HAND2 Antibody western blot of K562 cell line lysates (35 ug/lane). The HAND2 antibody detected the HAND2 protein (arrow).

Requested From: 
Date Requested: 10/20/2017

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