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Anti-DENND1B Antibody (aa144-193) IHC-plus™ LS-B5833

Note: This antibody replaces LS-C102467

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular DENND1B Antibodies

Anti-DENND1B Antibody (aa108-157) IHC-plus™ LS-B5832
Rabbit Polyclonal (IgG) to Human DENND1B
Human, Bovine, Dog, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-DENND1B Antibody (aa144-193) IHC-plus™ LS-B5833
Rabbit Polyclonal (IgG) to Human DENND1B
Human, Monkey, Mouse, Bat, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-DENND1B Antibody (aa46-76, AP) LS-C234525
Rabbit Polyclonal (IgG) to Human DENND1B
Human
IHC, Western blot, Flow Cytometry, ELISA
AP Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human DENND1B
Human, Monkey, Mouse, Bat, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human DENND1B
Rabbit
Human, Monkey, Mouse, Bat, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Bovine (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

DENND1B antibody was raised against synthetic peptide located between aa144-193 of human DENND1B (Q6P3S1, NP_659414). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Dog, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Stickleback, Zebrafish (100%); Bovine (92%); Rat, Panda, Lizard, Xenopus, Beetle (83%).
LS-E19011 - Lyophilized - 100 µg - $145.00
aa144-193
Human DENND1B
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About DENND1B

Q6P3S1 NM_144977 NP_659414.2

DENND1B Antibody, C1ORF18 Antibody, C1orf218 Antibody, Connecdenn 2 Antibody, DENN/MADD domain containing 1B Antibody, Protein FAM31B Antibody, FAM31B Antibody, RP11-53I24.2 Antibody

Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).

Immunohistochemistry

Immunohistochemistry
Anti-DENND1B antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5833 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
DENND1B antibody LS-B5833 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/10/2016

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