(tested or 100% immunogen sequence identity)
Also available Ready-to-Use
Cytoskeletal preparation extracted from human ectocervical epithelium.
This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this mAb serves as differentiation-related marker of all stratified epithelia. it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas. Optimal dilution of the Cytokeratin 10 + 13 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
0.01% BSA, 0.05% sodium azide