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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CYP21A2 Antibody (aa59‑87) LS‑C97494
CYP21A2 antibody LS-C97494 is an unconjugated rabbit polyclonal antibody to human CYP21A2. Validated for WB.
Catalog
Size
Price
LS-C97494-400
400 µl
Unavailable

Popular CYP21A2 Products

Anti-CYP21A2 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-CYP21A2 antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9758 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Immunohistochemical analysis of Cytochrome P450 21A2 staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Western blot of CYP21A2 antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

CYP21A2 antibody LS-C97494 is an unconjugated rabbit polyclonal antibody to human CYP21A2. Validated for WB.
About CYP21A2
CYP21A2 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates steroids at the 21 position. Its activity is required for the synthesis of steroid hormones including cortisol and aldosterone. Mutations in this gene cause congenital adrenal hyperplasia. P08686 NM_000500 NP_000491.4

CYP21A2 Antibody, 21-OHase Antibody, CAH1 Antibody, CYP21B Antibody, Cytochrome P450-C21 Antibody, Cytochrome P450 21 Antibody, Cytochrome P450 XXI Antibody, p450c21B Antibody, CA21H Antibody, CYP21 Antibody, Cytochrome P-450c21 Antibody, Cytochrome P450-C21B Antibody, Steroid 21-hydroxylase Antibody, Steroid 21-monooxygenase Antibody


Specifications

Target
Human CYP21A2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • Western blot (1:1000)
Blocking Peptide
LS-E9341 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C97494. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa59-87
Specificity
This CYP21A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-87 amino acids from the N-terminal region of human CYP21A2.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of CYP21A2 Antibody in 293, HL-60 cell line lysates (35 ug/lane). CYP21A2 (arrow) was detected using the purified antibody.
Western blot of CYP21A2 Antibody in 293, HL-60 cell line lysates (35 ug/lane). CYP21A2 (arrow) was detected using the purified antibody.

Western blot

Western blot of CYP21A2 Antibody in 293, HL-60 cell line lysates (35 ug/lane). CYP21A2 (arrow) was detected using the purified antibody.
Western blot of CYP21A2 Antibody in 293, HL-60 cell line lysates (35 ug/lane). CYP21A2 (arrow) was detected using the purified antibody.

Western blot

Western blot of CYP21A2 Antibody in 293, HL-60 cell line lysates (35 ug/lane). CYP21A2 (arrow) was detected using the purified antibody.
Western blot of CYP21A2 Antibody in 293, HL-60 cell line lysates (35 ug/lane). CYP21A2 (arrow) was detected using the purified antibody.

Western blot

Western blot of CYP21A2 Antibody in 293, HL-60 cell line lysates (35 ug/lane). CYP21A2 (arrow) was detected using the purified antibody.
Western blot of CYP21A2 Antibody in 293, HL-60 cell line lysates (35 ug/lane). CYP21A2 (arrow) was detected using the purified antibody.


Requested From: 
Date Requested: 6/22/2018

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