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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CYLD Antibody (aa364‑378) IHC‑plus™ LS‑B4323
Note: This antibody replaces LS-C54691
CYLD antibody LS-B4323 is an unconjugated goat polyclonal antibody to CYLD from mouse, rat and hamster. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B4323-50
50 µg (0.5 mg/ml)
Unavailable

Popular CYLD Products

Antibody
Species: Human, Monkey, Mouse, Bovine, Dog, Hamster, Horse, Rabbit
Applications: ELISA
ICC staining of HeLa cell using control (rabbit Ig) at 5 ug/ml.
Species: Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit, Chicken
Applications: ICC, Western blot
WB using CYLD Antibody (733)
Species: Human, Mouse, Rat, Bovine, Horse, Primate
Applications: ICC, Immunofluorescence, Western blot
CYLD antibody Western blot. All lanes: Anti CYLD at 0.5 ug/ml. Lane 1: Rat Testis Tissue Lysate at 50 ug. Lane 2: Rat Brain Tissue Lysate at 50 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Predicted band size: 107 kD. Observed band size: 107 kD.
Species: Human, Rat, Bovine, Horse, Pig, Sheep
Applications: Western blot

Product Description

CYLD antibody LS-B4323 is an unconjugated goat polyclonal antibody to CYLD from mouse, rat and hamster. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About CYLD
Protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Has endodeubiquitinase activity. Plays an important role in the regulation of pathways leading to NF-kappa-B activation. Contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation. Negative regulator of Wnt signaling. Inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules. Q9NQC7 NM_015247 CAB93533.1

CYLD Antibody, CDMT Antibody, Deubiquitinating enzyme CYLD Antibody, CYLDI Antibody, EAC Antibody, HSPC057 Antibody, MFT1 Antibody, MFT Antibody, SBS Antibody, TEM Antibody, BRSS Antibody, Ubiquitin thiolesterase CYLD Antibody, USPL2 Antibody, CYLD1 Antibody, KIAA0849 Antibody, Ubiquitin thioesterase CYLD Antibody


Specifications

Target
Mouse CYLD
Host
Goat
Reactivity
Mouse, Rat, Hamster (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:32000) & (0.5 - 1.5 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CYLD antibody was raised against synthetic peptide C-DSQQSKSKNPWYIDE from an internal region of mouse CYLD (NP_775545.1). Percent identity by BLAST analysis: Mouse, Hamster (100%).
Blocking Peptide
LS-E26305 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B4323. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa364-378
Specificity
Mouse CYLD.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-CYLD antibody IHC of mouse brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4323 concentration 5 ug/ml.
Anti-CYLD antibody IHC of mouse brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4323 concentration 5 ug/ml.

Western blot

Antibody (0.5 ug/ml) staining of rat brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.5 ug/ml) staining of rat brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-CYLD antibody IHC of mouse brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4323 concentration 5 ug/ml.
Anti-CYLD antibody IHC of mouse brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4323 concentration 5 ug/ml.

Western blot

Antibody (0.5 ug/ml) staining of rat brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.5 ug/ml) staining of rat brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-CYLD antibody IHC of mouse brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4323 concentration 5 ug/ml.
Anti-CYLD antibody IHC of mouse brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4323 concentration 5 ug/ml.

Western blot

Antibody (0.5 ug/ml) staining of rat brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.5 ug/ml) staining of rat brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-CYLD antibody IHC of mouse brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4323 concentration 5 ug/ml.
Anti-CYLD antibody IHC of mouse brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4323 concentration 5 ug/ml.

Western blot

Antibody (0.5 ug/ml) staining of rat brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.5 ug/ml) staining of rat brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/18/2018

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