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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CYBA / p22phox Antibody (aa117‑145) LS‑C167196
P22phox antibody LS-C167196 is an unconjugated rabbit polyclonal antibody to human p22phox (CYBA). Validated for IF and WB.
Catalog
Size
Price
LS-C167196-400
400 µl
Unavailable

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Product Description

P22phox antibody LS-C167196 is an unconjugated rabbit polyclonal antibody to human p22phox (CYBA). Validated for IF and WB.
About CYBA / p22phox
Cytochrome b is composed of cytochrome b alpha (CYBA; flavocytochrome b-558) and beta (CYBB; flavocytochrome b-245) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBA deficiency is associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. P13498 NM_000101 NP_000092.2

CYBA Antibody, Cytochrome b(558) alpha chain Antibody, Cytochrome b-245 light chain Antibody, Cytochrome b558 subunit alpha Antibody, p22 phagocyte B-cytochrome Antibody, p22-PHOX Antibody, Cytochrome b light chain Antibody, p22phox Antibody


Specifications

Target
Human CYBA / p22phox
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Blocking Peptide
LS-E1446 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C167196. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa117-145
Specificity
This CYBA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-145 amino acids from the C-terminal region of human CYBA.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Confocal immunofluorescence of CYBA Antibody with NCI-H292 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of CYBA Antibody with NCI-H292 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

CYBA Antibody western blot of NCI-H292 cell line lysates (35 ug/lane). The CYBA antibody detected the CYBA protein (arrow).
CYBA Antibody western blot of NCI-H292 cell line lysates (35 ug/lane). The CYBA antibody detected the CYBA protein (arrow).

Immunofluorescence

Confocal immunofluorescence of CYBA Antibody with NCI-H292 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of CYBA Antibody with NCI-H292 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

CYBA Antibody western blot of NCI-H292 cell line lysates (35 ug/lane). The CYBA antibody detected the CYBA protein (arrow).
CYBA Antibody western blot of NCI-H292 cell line lysates (35 ug/lane). The CYBA antibody detected the CYBA protein (arrow).

Immunofluorescence

Confocal immunofluorescence of CYBA Antibody with NCI-H292 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of CYBA Antibody with NCI-H292 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

CYBA Antibody western blot of NCI-H292 cell line lysates (35 ug/lane). The CYBA antibody detected the CYBA protein (arrow).
CYBA Antibody western blot of NCI-H292 cell line lysates (35 ug/lane). The CYBA antibody detected the CYBA protein (arrow).

Immunofluorescence

Confocal immunofluorescence of CYBA Antibody with NCI-H292 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of CYBA Antibody with NCI-H292 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

CYBA Antibody western blot of NCI-H292 cell line lysates (35 ug/lane). The CYBA antibody detected the CYBA protein (arrow).
CYBA Antibody western blot of NCI-H292 cell line lysates (35 ug/lane). The CYBA antibody detected the CYBA protein (arrow).


Requested From: 
Date Requested: 6/22/2018

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