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Anti-CUL3 / Cullin 3 Antibody (N-Terminus) IHC-plus™ LS-B359

Note: This antibody replaces LS-C18988

Ordering

Wt. Vol. Conc. Price
- 50 µl 85 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
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Most Popular CUL3 / Cullin 3 Antibodies

Anti-CUL3 / Cullin 3 Antibody (N-Terminus) IHC-plus™ LS-B359
Rabbit Polyclonal to Human CUL3 / Cullin 3
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CUL3 / Cullin 3 Antibody LS-C63987
Mouse Monoclonal (IgG1) to Human CUL3 / Cullin 3
Human, Mouse, Rat
ICC, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
Anti-CUL3 / Cullin 3 Antibody (clone 2236C1a) LS-C184383
Mouse Monoclonal [clone 2236C1a] (IgG1) to Human CUL3 / Cullin 3
Human, Mouse, Rat
ICC, Western blot, Immunoprecipitation, Flow Cytometry, Dot Blot
Unconjugated
Immunocytochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (2)
Rabbit Polyclonal to Human CUL3 / Cullin 3
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human CUL3 / Cullin 3
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Delipidated and defibrinated
Unmodified

Applications

  • IHC - Paraffin (1:500)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
  • ELISA (1:2000 - 1:10000)

Specificity and Use

CUL3 / Cullin 3 antibody was raised against synthetic peptide from human CUL3 / Cullin 3.
N-Terminus
Amino acids 1-12 of Human Cul3 (N-terminus) coupled to KLH.
Immunohistochemistry: LS-B359 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B359 was determined to be 1:500.

Packaging

0.01% sodium azide
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

Publications (2)

1
cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family. Kipreos ET, Lander LE, Wing JP, He WW, Hedgecock EM. Cell. 1996 85:829-39. [PubMed:8681378]
2
Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin ligases. Furukawa M, He YJ, Borchers C, Xiong Y. Nature cell biology. 2003 5:1001-7. [PubMed:14528312]

About CUL3 / Cullin 3

Q13618 NP_003581.1

CUL3 Antibody, Cullin 3 Antibody, Cullin homolog 3 Antibody, Cullin-3 Antibody, KIAA0617 Antibody, PHA2E Antibody, CUL-3 Antibody

Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme.

Immunohistochemistry

Immunohistochemistry
Anti-CUL3 / Cullin 3 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B359 dilution 1:500.
 

Requested From: United States
Date Requested: 12/6/2016

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