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Anti-CUL2 / Cullin 2 Antibody (aa748-759) IHC-plus™ LS-B8334

Note: This antibody replaces LS-C113001
Catalog Size Price
LS-B8334-50 50 µg (0.5 mg/ml) Unavailable

Most Popular CUL2 / Cullin 2 Antibodies

Anti-CUL2 / Cullin 2 Antibody (C-Terminus) IHC-plus™ LS-B358
Rabbit Polyclonal to Human CUL2 / Cullin 2
Human
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-CUL2 / Cullin 2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B358 dilution 1:500.
Anti-CUL2 / Cullin 2 Antibody (aa696-745) IHC-plus™ LS-B7019
Rabbit Polyclonal (IgG) to Human CUL2 / Cullin 2
Human, Mouse
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Anti-CUL2 / Cullin 2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7019 dilution 1:100.
Anti-CUL2 / Cullin 2 Antibody (aa748-759) IHC-plus™ LS-B8334
Goat Polyclonal to Human CUL2 / Cullin 2
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit, Chicken, Xenopus
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Anti-Cullin 2 / CUL2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8334 dilution 3.75 ug/ml.
Anti-CUL2 / Cullin 2 Antibody (C-Terminus) LS-C353051
Rabbit Polyclonal to Human CUL2 / Cullin 2
Human, Mouse, Rat, Bovine, Dog, Pig, Chicken
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemical analysis of Cullin 2 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Anti-CUL2 / Cullin 2 Antibody LS-C415840
Rabbit Polyclonal to Human CUL2 / Cullin 2
Human, Mouse
IHC, ICC, Immunofluorescence, Western blot
Unconjugated
Western blot of LOVO cell lysate using Cullin 2 Antibody

100% Guaranteed
Goat Polyclonal to Human CUL2 / Cullin 2
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit, Chicken, Xenopus
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated

Details

Human CUL2 / Cullin 2
Goat
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit, Chicken, Xenopus (tested or 100% immunogen sequence identity)
Hamster, Zebrafish (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)

Specificity and Use

CUL2 / Cullin 2 antibody was raised against synthetic peptide C-QYIERSQASADE from the C-terminus of human CUL2 / Cullin 2 (NP_003582.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus (100%); Hamster, Seabass, Stickleback, Pufferfish, Zebrafish (92%).
LS-E27376 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B8334. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa748-759
Human CUL2 / Cullin 2.
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 100kDa band observed in lysates of cell line NIH3T3 (calculated MW of 87.0kDa according to Human NP_003582.2 and of 86.9kDa according to Mouse NP_083678.2). Recommended concentration: 1-3 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About CUL2 / Cullin 2

Q13617 NM_003591 NP_003582.2

CUL2 Antibody, Cullin homolog 2 Antibody, CUL-2 Antibody, Cullin 2 Antibody, Cullin-2 Antibody

Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme.

Immunohistochemistry

Anti-Cullin 2 / CUL2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8334 dilution 3.75 ug/ml.
Anti-Cullin 2 / CUL2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8334 dilution 3.75 ug/ml.

Western blot

CUL2 antibody (1 ug/ml) staining of NIH3T3 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CUL2 antibody (1 ug/ml) staining of NIH3T3 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 5/20/2018

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