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Anti-CSTF2 / CstF-64 Antibody (aa309-320) LS-C204975

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular CSTF2 / CstF-64 Antibodies

Anti-CSTF2 / CstF-64 Antibody (aa182-231) IHC-plus™ LS-B12593
Rabbit Polyclonal (IgG) to Human CSTF2 / CstF-64
Human, Monkey
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image
Anti-CSTF2 / CstF-64 Antibody (N-Terminus) IHC-plus™ LS-B12594
Rabbit Polyclonal to Mouse CSTF2 / CstF-64
Mouse, Human, Monkey, Rat, Bovine, Dog, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Mouse CSTF2 / CstF-64
Mouse, Bat, Pig
ELISA
Unconjugated

Details

Mouse CSTF2 / CstF-64
Goat
Mouse, Bat, Pig (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

ELISA (1:128000)

Specificity and Use

CSTF2 / CstF-64 antibody was raised against synthetic peptide C-PASIERVQGQRT from an internal region of mouse CSTF2 / CstF-64 (ACF05702.1; ACF05700.1). Percent identity by BLAST analysis: Mouse, Bat, Pig (100%); Ferret (92%); Human (67%).
aa309-320
Mouse CSTF2 / CstF-64. This antibody recognizes mouse variants 1 and 3 of the reported beta isoform (ACF05702.1; ACF05700.1).
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Immunoprecipitation: In transfected HeLa transiently expressing Mouse [Character df]Cstf-64 ((DYKDDDDK tagged), a band of approx. 80kD was observed using anti-DYKDDDDK antibody in Western blot after immunoprecipitation with EB11838. No bands were observed in mock-transfected HeLa. Yields were similar between RIPA buffer and Triton X-100 buffer conditions. Recommended amount, 2 ug per lysate. Immunoprecipitation: An approx 75kD band is observed when endogenously expressed [Character df]Cstf-64 is pulled down from Mouse Brain lysates using EB11838, while Cstf-64 which is endogenously expressed in Mouse Liver, is not pulled down. Western blot was probed with a mouse monoclonal antibody recognizing both [Character df]Cstf-64 anf Cstf-64 Recommended amount 2 ug EB11838/500 ug lysate protein.

Packaging

Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About CSTF2 / CstF-64

P33240 NM_001325 NP_001316.1

CSTF2 Antibody, BetaCstF-64 variant 2 Antibody, CstF-64 Antibody, CSTF 64 kDa subunit Antibody, CF-1 64 kDa subunit Antibody

One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs.

Western blot

Western blot
CSTF2 antibody (2 ug) used to pull down [Character df]Cstf-64 from Mouse Brain (lane 5) lysate (500 ug protein) while it cannot pull down Cstf-64 from Mouse Liver (lane 6) lysate (500 ug) protein) using Protein G-coated magnetic beads. A 1/40th part of the lysates were loaded in lanes 1 (Brain) and 2 (Liver) before IP and in lanes 3 (Brain) and 4 (Liver) after IP. Western blot probed with mouse anti-[Character df]Cstf-64/Cstf-64 antibody and detected by chemiluminescence. Data obtained from Dr P Grozdanov, Texas Tech University Health Sciences Center, USA.

Western blot

Western blot
CSTF2 antibody HeLa lysate either in RIPA buffer (lanes 1, 2, 4, 6) or TX100 buffer (lanes 3, 5, 7) overexpressing Mouse [Character df]Cstf-64 (DYKDDDDK tagged) immunoprecipitated using (2 ug) with Protein G-coated magnetic beads in lanes 6 and 7 and probed with anti-DYKDDDDK (1/1000). Mock-transfected HeLa in Lane 1. A 1/40th part of the lysate was loaded in lanes 2 and 3 before IP and in lanes 4 an 5 after IP. Primary incubations were for 1 hour. Detected by chemiluminescence. Data obtained from Dr P Grozdanov, Texas Tech University Health Sciences Center, USA.

Requested From: United States
Date Requested: 12/11/2016

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