Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
IgG3 Monoclonal [Cyst13]
Protein A purified
Specificity and Use
KLH-PEG with terminal methoxy group was used as an immunogen. This recognizes the terminal methoxy group of the PEG molecule. Anti-PEG-47-Biotin is prepared by the biotinylation of the via primary amines.
This detects human Cystatin C, a member of the type 2 cystatin superfamily. Cystatin C is a potent inhibitor of cysteine proteinases, both endogenous (lysosomal cathepsins), and pathogenic.
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens.
WB: Cystatin C Antibody - different monoclonal antibodies after Tricine-SDS-PAGE in reducing conditions. Lane 1: MAb Cyst13 Lane 2: MAb Cyst18 Lane 3: MAb Cyst19 Antigen: Cystatin C purified from human urine (RDI), 0.2 ug/lane
Enzyme-Linked Immunosorbent Assay
Calibration curves: cystatin C Antibody - One-step fluoroimmunoassay in streptavidin coated plates. Capture MAbs: Cyst24 and Cyst23 are biotinylated (200 ng/well). Detection MAbs: Cyst10, Cyst19, Cyst28 or Cyst13 are Eu3+- labeled (200 ng/ml). Incubation volume 100 ul.