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Anti-CSF2 / GM-CSF Antibody LS-C104671
Note: This antibody replaces LS-C54049, LS-C42960Most Popular CSF2 / GM-CSF Antibodies
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Details
| Human CSF2 / GM-CSF | |
| Rabbit | |
| Human (tested or 100% immunogen sequence identity) | |
| Polyclonal | |
| Unconjugated. Also available conjugated with Biotin. | |
| Immunoaffinity purified | |
| Unmodified |
Applications
- IHC
- Western blot
- ELISA (0.5 - 2 µg/ml)
- Neutralization (0.3 - 0.5 µg/ml)
- (applications tested for the base form of this product only)
Specificity and Use
| CSF2 / GM-CSF antibody was raised against e.coli derived recombinant Human GM-CSF. | |
| Human GM-CSF | |
| Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.25 ug/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hGM-CSF (1 ng/ml), a concentration of 0.13-0.20 ug/ml of this antibody is required. ELISA: To detect hGM-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human GM-CSF (LS-C104714) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hGM-CSF. Western Blot: To detect hGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGM-CSF is 1.5-3 ng/lane, under either reducing or non-reducing conditions. |
Packaging
| Lyophilized from PBS, pH 7.2 | |
| Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. | |
| Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles. | |
| For research use only. |
Publications (1)
1
Chemotherapy-Derived Inflammatory Responses Accelerate the Formation of Immunosuppressive Myeloid Cells in the Tissue Microenvironment of Human Pancreatic Cancer. Takeuchi S, Baghdadi M, Tsuchikawa T, Wada H, Nakamura T, Abe H, Nakanishi S, Usui Y, Higuchi K, Takahashi M, Inoko K, Sato S, Takano H, Shichinohe T, Seino K, Hirano S. Cancer research. 2015 75:2629-40. (IHC; Human)
[Full Text Article]
[PubMed:25952647]
About CSF2 / GM-CSF
P04141
NM_000758
NP_000749.2
CSF2 Antibody, Colony-stimulating factor Antibody, CSF Antibody, GM-CSF Antibody, Sargramostim Antibody, Molgramostin Antibody, GMCSF Antibody
CSF2 / GM-CSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.
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Requested From:
Date Requested: 4/25/2018
Date Requested: 4/25/2018
