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Anti-CSF2 / GM-CSF Antibody LS-C104671

Note: This antibody replaces LS-C54049, LS-C42960
Catalog Size Price
LS-C104671-50 50 µg Unavailable
LS-C104671-100 100 µg Unavailable

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144 CSF2 / GM-CSF Antibodies

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Anti-CSF2 / GM-CSF Antibody (clone 3209 (30-4)) LS-C41956
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Anti-CSF2 / GM-CSF Antibody LS-C104671
Rabbit Polyclonal to Human CSF2 / GM-CSF
Human
IHC, Western blot, ELISA, Neutralization
Unconjugated
IHC of GM-CSF antibody LS-C104671.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CSF2 / GM-CSF Antibody LS-C123657
Rat Monoclonal (IgG2a) to Mouse CSF2 / GM-CSF
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Anti-CSF2 / GM-CSF Antibody (aa59-85) LS-C167081
Rabbit Polyclonal to Human CSF2 / GM-CSF
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IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
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CSF2 antibody immunohistochemistry of formalin-fixed and paraffin-embedded human lung carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.

100% Guaranteed Publications (1)
Rabbit Polyclonal to Human CSF2 / GM-CSF
Human
IHC, Western blot, ELISA, Neutralization (applications tested for the base form of this product only)
Unconjugated

Details

Human CSF2 / GM-CSF
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin.
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • Western blot
  • ELISA (0.5 - 2 µg/ml)
  • Neutralization (0.3 - 0.5 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

CSF2 / GM-CSF antibody was raised against e.coli derived recombinant Human GM-CSF.
Human GM-CSF
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.25 ug/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hGM-CSF (1 ng/ml), a concentration of 0.13-0.20 ug/ml of this antibody is required. ELISA: To detect hGM-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human GM-CSF (LS-C104714) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hGM-CSF. Western Blot: To detect hGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGM-CSF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

Publications (1)

1
Chemotherapy-Derived Inflammatory Responses Accelerate the Formation of Immunosuppressive Myeloid Cells in the Tissue Microenvironment of Human Pancreatic Cancer. Takeuchi S, Baghdadi M, Tsuchikawa T, Wada H, Nakamura T, Abe H, Nakanishi S, Usui Y, Higuchi K, Takahashi M, Inoko K, Sato S, Takano H, Shichinohe T, Seino K, Hirano S. Cancer research. 2015 75:2629-40. (IHC; Human) [Full Text Article] [PubMed:25952647]

About CSF2 / GM-CSF

P04141 NM_000758 NP_000749.2

CSF2 Antibody, Colony-stimulating factor Antibody, CSF Antibody, GM-CSF Antibody, Sargramostim Antibody, Molgramostin Antibody, GMCSF Antibody

CSF2 / GM-CSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.

Immunohistochemistry

IHC of GM-CSF antibody LS-C104671.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC of GM-CSF antibody LS-C104671. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of GM-CSF antibody LS-C104671.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of GM-CSF antibody LS-C104671. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of GM-CSF antibody LS-C104671.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of GM-CSF antibody LS-C104671. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of GM-CSF antibody LS-C104671.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of GM-CSF antibody LS-C104671. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 4/25/2018

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