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Anti-CSF2 / GM-CSF Antibody LS-C104671
Note: This antibody replaces LS-C54049, LS-C42960Most Popular CSF2 / GM-CSF Antibodies
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Details
| Human CSF2 / GM-CSF | |
| Rabbit | |
| Human (tested or 100% immunogen sequence identity) | |
| Polyclonal | |
| Unconjugated. Also available conjugated with Biotin. | |
| Immunoaffinity purified | |
| Unmodified |
Applications
- IHC
- Western blot
- ELISA (0.5 - 2 µg/ml)
- Neutralization (0.3 - 0.5 µg/ml)
- (applications tested for the base form of this product only)
Specificity and Use
| CSF2 / GM-CSF antibody was raised against e.coli derived recombinant Human GM-CSF. | |
| Human GM-CSF | |
| Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.25 ug/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hGM-CSF (1 ng/ml), a concentration of 0.13-0.20 ug/ml of this antibody is required. ELISA: To detect hGM-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human GM-CSF (LS-C104714) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hGM-CSF. Western Blot: To detect hGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGM-CSF is 1.5-3 ng/lane, under either reducing or non-reducing conditions. |
Packaging
| Lyophilized from PBS, pH 7.2 | |
| Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. | |
| Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles. | |
| For research use only. |
About CSF2 / GM-CSF
P04141
NM_000758
NP_000749.2
CSF2 Antibody, Colony-stimulating factor Antibody, CSF Antibody, GM-CSF Antibody, Sargramostim Antibody, Molgramostin Antibody, GMCSF Antibody
CSF2 / GM-CSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.
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Requested From:
Date Requested: 8/16/2017
Date Requested: 8/16/2017
