Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 6B9H2] (IgG2b) to Human CSF1R / CD115 / FMS
IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Rat Monoclonal [clone AFS98] (IgG2a,k) to Mouse CSF1R / CD115 / FMS
Mouse CSF1R / CD115 / FMS
Mouse (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [AFS98]
Specificity and Use
The AFS98 antibody has been tested by flow cytometric analysis of peritoneal exudate cells. This can be used at less than or equal to 0.06 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, pH 7.2, 150 mM sodium chloride, 0.09% sodium azide
Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes.
Staining of C57Bl/6 peritoneal exudate cells with 0.03 ug PE Rat IgG2a isotype control (open histogram) or 0.03 ug PE anti-mouse CD115 (AFS98) (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.