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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CSF1R / CD115 / FMS Antibody (aa700‑710) LS‑C139625
FMS antibody LS-C139625 is an unconjugated goat polyclonal antibody to FMS (CSF1R / CD115) from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C139625-100
100 µg (0.5 mg/ml)
Unavailable

Popular CSF1R / CD115 / FMS Products

Anti-MCSF Receptor antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1070 concentration 10 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin
Staining of thioglycollate-induced PEC cells with 0.25 ug of Purified Rat IgG2a isotype control (open histogram) or 0.25 ug of Purified anti-mouse CD115 (AFS98) (colored histogram) followed by Biotin Anti-Rat IgG and SAv-PE. Total viable cells were used for analysis.
Species: Mouse
Applications: IHC, IHC - Frozen, Western blot, Flow Cytometry
Antibody
Species: Human
Applications: Flow Cytometry
Mouse Csf1r Antibody immunohistochemistry of formalin-fixed and paraffin-embedded mouse cerebellum tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Species: Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
IHC of paraffin-embedded prostate cancer tissues using CSF1R mouse monoclonal antibody with DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Flow Cytometry, ELISA

Product Description

FMS antibody LS-C139625 is an unconjugated goat polyclonal antibody to FMS (CSF1R / CD115) from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.
About CSF1R / CD115 / FMS
Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. P07333 NM_005211 NP_005202.2

CSF1R Antibody, CD115 antigen Antibody, CSF-1R Antibody, CSF-1-R Antibody, CSF-1 receptor Antibody, FMS Antibody, Fim-2 Antibody, FIM2 Antibody, FMS proto-oncogene Antibody, M-CSFR Antibody, HDLS Antibody, M-cfs receptor Antibody, C-FMS Antibody, CD115 Antibody, CSF1 receptor Antibody, CSFR Antibody, M-CSF-R Antibody, MCSF Receptor Antibody, Proto-oncogene c-Fms Antibody


Specifications

Target
Human CSF1R / CD115 / FMS
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Dog, Hamster, Horse (tested or 100% immunogen sequence identity)
Predicted
Bovine (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 0.5 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Immunogen
Synthetic peptide C-KNIHLEKKYVR from an internal region of human CSF1R / MCSF Receptor (NP_005202.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Panda, Dog, Bat, Cat, Horse (100%); Elephant, Bovine (91%); Dolphin, Pig, Turkey, Zebra finch, Chicken, Platypus (82%).
Blocking Peptide
LS-E27447 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C139625. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa700-710
Specificity
Human CSF1R / MCSF Receptor.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: In transfected HEK293 transiently expressing full-length Human CSF1R (myc and DYKDDDDK tagged), a band of approx. 200kD was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.1-0.5 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CSF1R with C-terminal MYC tag probed with (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primar
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CSF1R with C-terminal MYC tag probed with (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primar

Western blot

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CSF1R with C-terminal MYC tag probed with (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primar
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CSF1R with C-terminal MYC tag probed with (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primar

Western blot

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CSF1R with C-terminal MYC tag probed with (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primar
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CSF1R with C-terminal MYC tag probed with (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primar

Western blot

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CSF1R with C-terminal MYC tag probed with (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primar
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CSF1R with C-terminal MYC tag probed with (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primar


Requested From: 
Date Requested: 6/21/2018

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