Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Protein G purified from tissue culture supernatant
IHC - Paraffin (10 µg/ml)
Western blot (1:2000)
(applications tested for the base form of this product only)
Specificity and Use
Native Alpha B Crystallin.
Detects a band at ~20kD (Predicted mol. weight is ~21 kD) corresponding to alpha B crystallin. Does not cross react with alpha A crystallin, beta-L crystallin, Beta-H crystallin, gamma crystallin, Hsp25, Hsp27 or Hsp47 proteins.
PBS, pH 7.2; 50% glycerol, 0.09% azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
CRYAB Antibody, Alpha-crystallin B chain Antibody, CRYA2 Antibody, Crystallin, alpha B Antibody, CTPP2 Antibody, Heat shock protein beta-5 Antibody, HSPB5 Antibody, Alpha B Crystallin Antibody, Alpha(B)-crystallin Antibody, Rosenthal fiber component Antibody, Heat-shock 20 kD like-protein Antibody
Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates.
Anti-CRYAB / Alpha B Crystallin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7454 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Alpha B Crystallin (3A10. C9), Cell lines. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Alpha B crystallin (3A10-C9), Rat tissues. This image was taken for the unconjugated form of this product. Other forms have not been tested.