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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CRYAB / Alpha B Crystallin Antibody (clone 2A1) LS‑C114647
Alpha B Crystallin antibody LS-C114647 is an unconjugated mouse monoclonal antibody to human Alpha B Crystallin (CRYAB). Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C114647-100
100 µl
Unavailable

Popular CRYAB / Alpha B Crystallin Products

Antibody
Species: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep
Applications: Western blot, Immunoprecipitation
Anti-CRYAB / Alpha B Crystallin antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3410 concentration 10 ug/ml.
Species: Bovine, Human, Mouse, Rat, Chicken
Applications: IHC, IHC - Paraffin, Western blot
Anti-CRYAB / Alpha B Crystallin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3696 concentration 10 ug/ml.
Species: Human, Mouse, Rat, Bovine, Pig
Applications: IHC, IHC - Paraffin, Western blot
Anti-CRYAB / Alpha B Crystallin antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7449 concentration 20 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Bovine
Applications: IHC, IHC - Paraffin, Western blot, ELISA
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).
Species: Human, Monkey, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry

Product Description

Alpha B Crystallin antibody LS-C114647 is an unconjugated mouse monoclonal antibody to human Alpha B Crystallin (CRYAB). Validated for Flow, IF, IHC and WB.
About CRYAB / Alpha B Crystallin
Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. P02511 NM_001885 NP_001876.1

CRYAB Antibody, Alpha-crystallin B chain Antibody, CRYA2 Antibody, Crystallin, alpha B Antibody, CTPP2 Antibody, Heat shock protein beta-5 Antibody, HSPB5 Antibody, Alpha B Crystallin Antibody, Alpha(B)-crystallin Antibody, Rosenthal fiber component Antibody, Heat-shock 20 kD like-protein Antibody


Specifications

Target
Human CRYAB / Alpha B Crystallin
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [2A1]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Full length human recombinant protein of human CRYAB (NP_001876) produced in HEK293T cell.
Specificity
Human CRYAB / Alpha B Crystallin
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded colon tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded colon tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded endometrium tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded endometrium tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded pancreas tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-CRYAB mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB.
Anti-CRYAB mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CRYAB antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CRYAB antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded colon tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded colon tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded endometrium tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded endometrium tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded pancreas tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-CRYAB mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB.
Anti-CRYAB mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CRYAB antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CRYAB antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded colon tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded colon tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded endometrium tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded endometrium tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded pancreas tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-CRYAB mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB.
Anti-CRYAB mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CRYAB antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CRYAB antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded colon tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded colon tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded endometrium tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded endometrium tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded pancreas tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-CRYAB mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB.
Anti-CRYAB mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CRYAB antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CRYAB antibody, and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/24/2018

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