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Anti-CORO1A / Coronin 1a Antibody (aa546-558) IHC-plus™ LS-B3234

Note: This antibody replaces LS-C54673
Catalog Size Price
LS-B3234-50 50 µg (0.5 mg/ml) Unavailable

Most Popular CORO1A / Coronin 1a Antibodies

Anti-CORO1A / Coronin 1a Antibody (N-Terminus) LS-C31093
Rabbit Polyclonal (IgG) to Human CORO1A / Coronin 1a
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Zebrafish
Western blot
Unconjugated
A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CORO1A / Coronin 1a Antibody (aa546-558) IHC-plus™ LS-B3234
Goat Polyclonal to Human CORO1A / Coronin 1a
Human, Monkey, Bovine, Dog, Horse
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Anti-CORO1A antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3234 concentration 75 ug/ml.
Anti-CORO1A / Coronin 1a Antibody IHC-plus™ LS-B10416
Rabbit Polyclonal to Human CORO1A / Coronin 1a
Human, Rat
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Anti-CORO1A / p57 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10416 dilution 1:100.
Anti-CORO1A / Coronin 1a Antibody (aa250-300) IHC-plus™ LS-B14784
Rabbit Polyclonal (IgG) to Human CORO1A / Coronin 1a
Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Detection of Human and Mouse Coronin 1 by Immunohistochemistry. Sample: FFPE section of human pancreatic islet cell tumor (left) and mouse fibroma (right). Antibody: Affinity purified rabbit anti-Coronin 1 used at a dilution of 1:200 (1 ug/ml) and 1:1000 (0.2 ug/ml). Detection: DAB.
Anti-CORO1A / Coronin 1a Antibody (aa7-204, Biotin) LS-C689148
Rabbit Polyclonal (IgG) to Human CORO1A / Coronin 1a
Human
IHC, Western blot
Biotin Conjugated
Western blot of recombinant CORO1A / p57.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

100% Guaranteed
Goat Polyclonal to Human CORO1A / Coronin 1a
Human, Monkey, Bovine, Dog, Horse
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated

Details

Human CORO1A / Coronin 1a
Goat
Human, Monkey, Bovine, Dog, Horse (tested or 100% immunogen sequence identity)
Mouse, Rat, Bat, Hamster (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (1:16000) & (0.25 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)

Specificity and Use

Synthetic peptide C-KRLDRLEETVQAK from the C-terminus of human CORO1A (NP_009005.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bovine, Horse (100%); Mouse, Rat, Hamster, Bat (92%); Rabbit (85%).
LS-E25585 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3234. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa546-558
Human CORO1A.
Immunohistochemistry: LS-B3234 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3234 was determined to be 3.75 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About CORO1A / Coronin 1a

P31146 NM_007074 NP_009005.1

CORO1A Antibody, Clipin-A Antibody, Coronin-like protein A Antibody, Coronin-like protein p57 Antibody, CLIPINA Antibody, Coronin-1 Antibody, Coronin-1A Antibody, HCORO1 Antibody, p57 Antibody, TACO Antibody, CLABP Antibody, CORO1 Antibody

May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes.

Immunohistochemistry

Anti-CORO1A antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3234 concentration 75 ug/ml.
Anti-CORO1A antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3234 concentration 75 ug/ml.

Immunohistochemistry

Anti-CORO1A antibody IHC of human small intestine, lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3234 concentration 75 ug/ml.
Anti-CORO1A antibody IHC of human small intestine, lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3234 concentration 75 ug/ml.

Western blot

HEK293 overexpressing CORO1A (RC210753) and probed with (mock transfection in first lane).
HEK293 overexpressing CORO1A (RC210753) and probed with (mock transfection in first lane).

Western blot

Antibody staining (0.3 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.3 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Requested From: 
Date Requested: 5/23/2018

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