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Anti-Complement C4 Antibody (clone 2.53E+04) LS-C22313


Wt. Vol. Conc. Price
- 100 µl - Unavailable

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Mouse Monoclonal [clone 2.53E+04] (IgG1,k) to Human Complement C4
Western blot, ELISA


Human Complement C4
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [2.53E+04]
Protein A/G purified


  • Western blot
  • ELISA (1:4000)

Specificity and Use

Complement C4 antibody was raised against c4 isolated from human plasma.
Has specificity for human C4. Epitope specificity differs from that of clone 6F70 as determined by inhibition ELISA. Reacts strongly with C4. Strong reaction is seen with normal human serum, and no reaction is seen with serum depleted of C4, when tested in sandwich ELISA in combination with a polyclonal antibody against C4. Also reacts with C4b deposited on coated antibody molecules. In Western blotting after SDS-PAGE reacts with C4 in reduced as well as non-reduced forms.
Suitable for use in ELISA, Western Blot. ELISA: 1:4000.


PBS, pH 7.2, 15 mM sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About Complement C4

Complement component 4 is a protein involved in the complement system. It is cleaved into proteins 4a and 4b; 4b can be further cleaved into 4c and 4d. C4a is an anaphylatoxin. C4b forms part of C3-convertase, in conjunction with 2a, and can bind CR1. C4d has been identified as a biomarker for systemic lupus erythematosus.

Requested From: 
Date Requested: 4/24/2017

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