Human Collagen VI

Goat

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Human type VI collagen.

Reacts with conformational determinants on bovine and human type VI collagen as demonstrated by ELISA. May react with type VI collagen from other species. Exhibits < 10% cross-reactivity with collagen type I, II, III, IV and V. Has not been tested for reactivity with other ECM proteins (e. g., laminin, fibronectin).

• IHC
• IHC - Paraffin (10 µg/ml)
• IHC - Frozen
• ICC (1:10 - 1:20)
• ELISA
• Dot Blot

Immunohistochemistry: LS-B3771 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3771 was determined to be 10 ug/ml.

Borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.