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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CLDN3 / Claudin 3 Antibody (phospho‑Tyr219) LS‑C198809
Claudin 3 antibody LS-C198809 is an unconjugated rabbit polyclonal antibody to human Claudin 3 (CLDN3). Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C198809-50
50 µl
Unavailable
LS-C198809-100
100 µl
Unavailable

Popular CLDN3 / Claudin 3 Products

Anti-CLDN3 / Claudin 3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2360 dilution 1:100.
Species: Mouse, Human, Rat
Applications: IHC, IHC - Paraffin
CLDN3 / Claudin 3 antibody LS-C117218 Western blot of Mouse Liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: Western blot
Anti-CLDN3 / Claudin 3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7017 dilution 1:100.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CLDN3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLDN3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLDN3.
Species: Human
Applications: Western blot

Product Description

Claudin 3 antibody LS-C198809 is an unconjugated rabbit polyclonal antibody to human Claudin 3 (CLDN3). Validated for Peptide-ELISA and WB.
About CLDN3 / Claudin 3
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. O15551 NM_001306 NP_001297.1

CLDN3 Antibody, C7orf1 Antibody, Claudin-3 Antibody, CPE-R 2 Antibody, Claudin 3 Antibody, RVP1 Antibody, CPE-R2 Antibody, CPE-receptor 2 Antibody, CPETR2 Antibody, HRVP1 Antibody


Specifications

Target
Human CLDN3 / Claudin 3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:1000)
Immunogen
Synthetic peptide derived from human Claudin 3 around the phosphorylation site of Tyr219.
Epitope
pTyr219
Specificity
Claudin 3 (Phospho-Tyr219) Antibody detects endogenous levels of Claudin 3 only when phosphorylated at tyrosine219.
Presentation
PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of extracts from COLO205 cells treated with EGF 200ng/ml 30', using Claudin 3 (Phospho-Tyr219) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from COLO205 cells treated with EGF 200ng/ml 30', using Claudin 3 (Phospho-Tyr219) Antibody. The lane on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from COLO205 cells treated with EGF 200ng/ml 30', using Claudin 3 (Phospho-Tyr219) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from COLO205 cells treated with EGF 200ng/ml 30', using Claudin 3 (Phospho-Tyr219) Antibody. The lane on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from COLO205 cells treated with EGF 200ng/ml 30', using Claudin 3 (Phospho-Tyr219) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from COLO205 cells treated with EGF 200ng/ml 30', using Claudin 3 (Phospho-Tyr219) Antibody. The lane on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from COLO205 cells treated with EGF 200ng/ml 30', using Claudin 3 (Phospho-Tyr219) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from COLO205 cells treated with EGF 200ng/ml 30', using Claudin 3 (Phospho-Tyr219) Antibody. The lane on the right was incubated with synthetic peptide.


Requested From: 
Date Requested: 6/21/2018

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