Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Citrulline antibody was raised against citrulline coupled to KLH via Glutaraldehyde
The antibody reacts specifically with intrapeptidic citrulline independently of AA sequence
The positive control was HL60 cells treated with DMSO or with DMSO and A23187. The histones of these cells were then extracted.The sample were loaded on a 14% acrylamide SDS PAGE gel.The transfer was made on a PVDF membrane The blocking buffer was PBS 1X with 5% BSA The antibody was used at 1/1000 in a TBT Tween 20 buffer with 1% BSA.The antibody was incubated overnight at +4°C.
100 µl Distilled water
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.