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Anti-CHRM3 / M3 Antibody (C-Terminus) LS-C37395

Ordering

Wt. Vol. Conc. Price
- 100 µl - $425
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CHRM3 / M3 Antibodies

Anti-CHRM3 / M3 Antibody (C-Terminus) IHC-plus™ LS-A5259
Rabbit Polyclonal to Human CHRM3 / M3
Human, Monkey
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-CHRM3 / M3 Antibody (Cytoplasmic Domain) IHC-plus™ LS-A1421
Rabbit Polyclonal to Human CHRM3 / M3
Human, Monkey
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CHRM3 / M3 Antibody (Cytoplasmic Domain) IHC-plus™ LS-A5385
Rabbit Polyclonal to Human CHRM3 / M3
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human CHRM3 / M3
Human
IHC, Western blot, ELISA
Unconjugated

Details

Human CHRM3 / M3
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

  • IHC
  • Western blot (1:500)
  • ELISA

Specificity and Use

CHRM3 / M3 antibody was raised against peptide analog of the carboxyl terminal of the muscarinic M3 receptor covalently attached onto a carrier protein.
C-Terminus
Recognizes rat M3 Muscarinic Receptor. Does not recognize M1 Muscarinic Receptor (451-460), M1 Muscarinic Receptor, M2 Muscarinic Receptor (457-466), M2 Muscarinic Receptor, M4 Muscarinic Receptor (469-478), M4 Muscarinic Receptor, M5 Muscarinic Receptor (519-531) and M5 Muscarinic Receptor. Specific for the C-terminal of the M3 receptor.
Western Blot: 1:500Immunohistochemistry: 1:2500 using PLP-fixed rat brain sections (ABC technique)Optimal dilutions to be determined by the researcher.Western Blot Protocol:1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels, such at 12% gels) and electrophoretic transfer to PVDFmembrane, block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer.2. Wash 2X with TBS/Tween-20.3. Apply LS-C37395 at dilution of at least 1:500 (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 asbuffer for the primary antibody. Incubate for 2-4 hours.4. Wash 3X with TBS/Tween-20.5. Apply affinity purified IgG (HRP) goat x rabbit antiserum (dilution may vary depending upon supplier) in 2% normal goat serum inTBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.6. Wash 4X for 5 minute/wash with TBS/Tween-20.7. Develop color using the enhanced DAB reaction.

Packaging

Lyophilized.
100 µl 100ul PBS, 10mg/ml BSA
Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid frequent freeze thaws.
For research use only.

About CHRM3 / M3

P20309 NM_000740 NP_000731.1

CHRM3 Antibody, M3 Antibody, Muscarinic receptor M3 Antibody, Muscarinic M3 receptor Antibody, EGBRS Antibody, HM3 Antibody, M3 muscarinic receptor Antibody

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system.

Requested From: United States
Date Requested: 12/6/2016

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