(tested or 100% immunogen sequence identity)
- IHC (5 µg/ml)
- Western blot (20 µg/ml)
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CHRM1 / M1 antibody was raised against peptide analogue of the carboxyl terminal of the M1 receptor covalently attached onto a carrier protein.
Recognizes human and rat Muscarinic Receptor M1. Cross-reactivity: M1 (aa451-460): 100%, M1 (full receptor): 60%. No reactivity is observed with M2-M5 (full receptors), M2 (aa457-466), M3 (aa580-589), M4 (aa469-478) or M5 (aa519-531).
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications have been tested. Western Blot: 20 ug/ml using whole rat brain homogenate. Immunohistochemistry: 5 ug/ml has been found to stain PLP fixed rat brain sections known to express the muscarinic M1 receptor using ABC techniques.
PBS, pH 7.2, 10 mg/ml BSA.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
For research use only.