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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CHRM1 / M1 Antibody (aa451‑460) LS‑C9130
M1 antibody LS-C9130 is an unconjugated rabbit polyclonal antibody to M1 (CHRM1) from human and rat. Validated for ELISA, IHC and WB.
Antibody
Catalog
Size
Price
LS-C9130-100
100 µl (1 mg/ml)
$375
LS-C9130-500
500 µl
$605

Popular CHRM1 / M1 Products

Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Antibody (1 ug/ml) staining of Human Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Species: Human, Monkey, Mouse, Rat, Bovine, Hamster, Horse, Pig, Rabbit
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Western blot analysis of lysates from HepG2 cells, using CHRM1 Antibody. The lane on the right is blocked with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Western blot analysis of extracts of various cell lines, using CHRM1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
Species: Human, Mouse, Rat
Applications: IHC, Western blot

Product Description

M1 antibody LS-C9130 is an unconjugated rabbit polyclonal antibody to M1 (CHRM1) from human and rat. Validated for ELISA, IHC and WB.
About CHRM1 / M1
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. P11229 NM_000738 NP_000729.2

CHRM1 Antibody, Acetylcholine receptor M1 Antibody, M1R Antibody, M1 Antibody, M1 muscarinic receptor Antibody, HM1 Antibody, Muscarinic M1 receptor Antibody


Specifications

Target
Human CHRM1 / M1
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC (5 µg/ml)
  • Western blot (20 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CHRM1 / M1 antibody was raised against peptide analogue of the carboxyl terminal of the M1 receptor covalently attached onto a carrier protein.
Epitope
aa451-460
Specificity
Recognizes human and rat Muscarinic Receptor M1. Cross-reactivity: M1 (aa451-460): 100%, M1 (full receptor): 60%. No reactivity is observed with M2-M5 (full receptors), M2 (aa457-466), M3 (aa580-589), M4 (aa469-478) or M5 (aa519-531).
Usage
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications have been tested. Western Blot: 20 ug/ml using whole rat brain homogenate. Immunohistochemistry: 5 ug/ml has been found to stain PLP fixed rat brain sections known to express the muscarinic M1 receptor using ABC techniques.
Presentation
PBS, pH 7.2, 10 mg/ml BSA.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only.

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Requested From: United States
Date Requested: 9/24/2018

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