Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
IHC (5 µg/ml)
Western blot (20 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
CHRM1 / M1 antibody was raised against peptide analogue of the carboxyl terminal of the M1 receptor covalently attached onto a carrier protein.
Recognizes human and rat Muscarinic Receptor M1. Cross-reactivity: M1 (aa451-460): 100%, M1 (full receptor): 60%. No reactivity is observed with M2-M5 (full receptors), M2 (aa457-466), M3 (aa580-589), M4 (aa469-478) or M5 (aa519-531).
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications have been tested. Western Blot: 20 ug/ml using whole rat brain homogenate. Immunohistochemistry: 5 ug/ml has been found to stain PLP fixed rat brain sections known to express the muscarinic M1 receptor using ABC techniques.
PBS, pH 7.2, 10 mg/ml BSA.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system.