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Anti-Chlamydia trachomatis Antibody (FITC) LS-C79163

Ordering

Wt. Vol. Conc. Price
- 1000 µl - $465
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular Chlamydia trachomatis Antibodies

Anti-Chlamydia trachomatis Antibody (FITC) LS-C53005
Mouse Monoclonal to Chlamydia trachomatis
Chlamydia trachomatis
IHC, ELISA
FITC Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Chlamydia trachomatis
Chlamydia trachomatis
Immunofluorescence, Functional Assay
FITC Conjugated

Details

Chlamydia trachomatis
Rabbit
Chlamydia trachomatis (tested or 100% immunogen sequence identity)
IgG Polyclonal
Fluorescein
Purified
Unmodified

Applications

  • Immunofluorescence
  • Functional Assay

Specificity and Use

L2 + other serovar groups.
Purified elementary bodies, disrupted. Cross-reacts with Chlamydia psittacii and Chlamydia pneumoniae (TWAR). Negative vs. HEp-2 cells and egg yolk sac.
Direct FA staining of target antigens in a permissive tissue culture system. Working dilution must be determined by the user for his or her application but a starting range of 1:10-1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. Titer is 1:1000-3000 by indirect immunofluorescence vs. all serovars (A-K, L1-L3). Chlamydia is caused by the bacterium Chlamydia trachomatis. The intracytoplasmic inclusions caused by the bacterium are draped around the infected cell's nucleus. Chlamydia has a genome size of approximately 500-1000 kilobases and contains both RNA and DNA. The organism is also extremely temperature sensitive and must be refrigerated at 4?C as soon as a sample is obtained. Colonization of Chlamydia begins with attachment to sialic acid receptors on the eye, throat or genitalia. It persists at body sites that are inaccessible to phagocytes, T-cells, and B-cells. It also exists as 15 different serotypes. These serotypes cause four major diseases in humans: endemic trachoma (caused by serotypes A and C), sexually transmitted disease and inclusion conjunctivitis (caused by serotypes D and K), and lymphogranuloma venereum (caused by serotypes L1, L2, and L3). Studies reveal that Chlamydia, because of its cell wall, is able to inhibit phagolysosome fusion in phagocytes. The cell wall is proposed to be gram-negative in that it contains an outer lipopolysaccharide membrane, but it lacks peptidoglycan in its cell wall. This lack of peptidoglycan is shown by the inability to detect muramic acid and antibodies directed against it. It may, however, contain a carboxylated sugar other than muramic acid. The proposed structure consists of a major outer membrane protein cross-linked with disulfide bonds. It also contain cysteine-rich proteins (CRP) that may be the functional equivalent to peptidoglycan. This unique structure allows for intracellular division and extracellular survival (Hatch 1996). -Kenneth Todar, University of Wisconsin-Madison. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

0.01 M PBS, pH 7.2, 10 mg/ml BSA, 0.1% sodium azide.
For research use only.

About Chlamydia trachomatis

Chlamydia trachomatis, an obligate intracellular human pathogen, is one of four bacterial species in the genus Chlamydia. C. trachomatis includes three human biovars: serovars Ab, B, Ba, or C cause trachoma: infection of the eyes, which can lead to blindness; serovars D-K cause urethritis, pelvic inflammatory disease, ectopic pregnancy, neonatal pneumonia, and neonatal conjunctivitis; serovars L1, L2 and L3 cause ymphogranuloma venereum (LGV).

Requested From: United States
Date Requested: 12/7/2016

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