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Anti-CFH / Complement Factor H Antibody (aa577-588) IHC-plus™ LS-B2585

Note: This antibody replaces LS-C54667, LS-C65824

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml Unavailable

Most Popular CFH / Complement Factor H Antibodies

Anti-CFH / Complement Factor H Antibody (aa577-588) IHC-plus™ LS-B2585
Goat Polyclonal to Human CFH / Complement Factor H
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CFH / Complement Factor H Antibody (clone OX-24) IHC-plus™ LS-B2889
Mouse Monoclonal [clone OX-24] (IgG1) to Human CFH / Complement Factor H
Human
IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CFH / Complement Factor H Antibody (clone OX-24) IHC-plus™ LS-B3648
Mouse Monoclonal [clone OX-24] (IgG1) to Human CFH / Complement Factor H
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human CFH / Complement Factor H
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human CFH / Complement Factor H
Goat
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.03 - 0.1 µg/ml)
  • ELISA (1:32000)

Specificity and Use

CFH / Complement Factor H antibody was raised against synthetic peptide C-HLVPDRKKDQYK from an internal region of human CFH / Factor H (NP_000177.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%).
LS-E25912 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2585. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa577-588
Human CFH / Factor H. This antibody is expected to recognize isoform a (NP_000177.2) only.
Immunohistochemistry: LS-B2585 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2585 was determined to be 2.5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About CFH / Complement Factor H

P08603 NM_000186 NP_000177.2

CFH Antibody, Adrenomedullin binding protein Antibody, AHUS1 Antibody, AMBP1 Antibody, ARMS1 Antibody, ARMD4 Antibody, Beta-1-H-globulin Antibody, Factor H Antibody, Factor H-like 1 Antibody, HF Antibody, HF2 Antibody, H factor 1 Antibody, H factor 2 (complement) Antibody, HUS Antibody, HF1 Antibody, Beta-1H Antibody, CFHL3 Antibody, Complement factor H Antibody, H factor 1 (complement) Antibody

CFH / Complement Factor H is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short consensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy.

Immunohistochemistry

Immunohistochemistry
Anti-Factor H antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2585 concentration 5 ug/ml.

Western blot

Western blot
Antibody staining (0.03 ug/ml) of human lung lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Requested From: 
Date Requested: 3/26/2017

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