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Anti-CENPE Antibody (clone 1H12) LS-C59522

Catalog Size Price
LS-C59522-100 100 µg Unavailable

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Anti-CENPE Antibody (clone 1H12) LS-C59522
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Anti-CENPE Antibody (clone 1H12) LS-C82256
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Anti-CENPE Antibody (clone CENP-E.1H12) LS-C137476
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Anti-CENPE Antibody (aa2222-2361) LS-C293287
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Anti-CENPE Antibody LS-C403059
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100% Guaranteed
Mouse Monoclonal [clone 1H12] to Human CENPE
Immunofluorescence, Western blot, Immunoprecipitation


Human (tested or 100% immunogen sequence identity)
Monoclonal [1H12]
Protein A purified


  • Immunofluorescence
  • Western blot
  • Immunoprecipitation

Specificity and Use

CENPE antibody was raised against full length recombinant protein corresponding to human CENP-E protein.
human CENP-E protein. This antibody reacts with CENP-E from human cells. Reactivity against homologs from other sources is not known
This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~320 kD corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Short term: -20°C; Long term: -20°C.
For research use only.


Q02224 NM_001813 NP_001804.2

CENPE Antibody, CENP-E Antibody, Centromere protein E Antibody, Centromere protein E (312kD) Antibody, Kinesin-related protein CENPE Antibody, PPP1R61 Antibody, KIF10 Antibody, Centromere protein E, 312kDa Antibody, Kinesin family member 10 Antibody

Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end-directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity.


Anti-CENPE Antibody - Immunofluorescence Microscopy. monoclonal anti-CENPE antibody was used to detect CENPE protein, visible as discrete nuclear dots on prometaphase and metaphase cells that relocate to the spindle midzone at anaphase (panel A). Interphase cells show no discrete staining (bottom left, panel B). HeLa cells were fixed in paraformaldehyde and stained using this primary antibody. Alexa Fluor 555 TM conjugated anti-Mouse antibody (red) was used for detection. DNA was stained using bis-benzimide (DAPI) (green). Personal Communication, Tim Yen, Fox Chase Cancer Center, Philadelphia, PA.

Requested From: 
Date Requested: 1/21/2018

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