Target
Silkworm Cecropin B
Reactivity
Silkworm
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity chromatography
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic full-length cecropin B corresponding to a modified sequence from that which is found in silkworm: Lys-Trp-Lys-Val-Phe-Lys-Lys-Ile-Glu-Lys-Val-Gly-Arg-Asn-Ile-Arg-Asn-Gly-Ile-Val-Lys-Ala-Gly-Pro-Ala-Ile-Ala-Val-Leu-Gly-Glu-Ala-Lys-Ala-Leu.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified cecropin B. Reactivity against other forms of cecropins has not yet been determined.
Applications
- Western blot (1:500 - 1:5000)
- Immunoprecipitation (1:100)
- ELISA (1:5000 - 1:20000)
Usage
Anti-cecropin B is useful for the detection of cecropin B in various immunological assays. This reagent has specific value for the study of cecropins in transgenic plants. The antibody may also recognize naturally occurring cecropin A. Cecropin B has been modified in some instances by substituting a single amino acid to render the molecule less susceptible to proteolytic cleavage.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide, 50% Glycerol
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee