Human CDYL

Goat

Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence C-SMLKYLQRKIDEF, from the C-Terminus of protein sequence according to NP_004815.3NP_001137442.1NP_001137443.1.

aa586-598

Human CDYL. This antibody is expected to recognize both eported isoforms NP_004815.3; NP_001137442.1; NP_001137443.1). Variants (NP_001137442.1; NP_001137443.1) encode the same isoform.

• Western blot (1 - 3 µg/ml)
• Peptide Enzyme-Linked Immunosorbent Assay (1:4000)

Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx 75 kDa band observed in Human Testes lysates (calculated MW of 66.5 kDa according to NP_004815.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lahn et al , Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8707-12; PMID: 12072557). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 26 kDa. This band was successfully blocked by incubation with the immunising peptide.

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9Y232 NCBI: NM_004824 NP_004815.3