Target
Human CDKN2B / p15 INK4b
Synonyms
CDKN2B | CDK inhibitory protein | CDK4B inhibitor | INK4B | MTS2 | Multiple tumor suppressor 2 | p14_INK4B | p15 | p14_CDK inhibitor | p15 CDK inhibitor | p15 INK4b | p15-INK4b | p15_INK4B | p14-INK4b | p15INK4b | CDK4I | MTS-2 | TP15
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-RQGARERGARP, from the C-Terminus of protein sequence according to NP_004927.2NP_511042.1.
Specificity
Human CDKN2B / p15 INK4b. This antibody is expected to recognize isoform 2 (NP_511042.1) only.
Applications
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 12+14kD bands observed in Human Gastrointestinal cancer lysates (calculated MW of 8.1kD according to NP_511042.1). The observed 14kD cannot represent isoform 1 and therefore it most likely represents another post translational modification of isoform 2. Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee