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Anti-CDKN2A / p16INK4a Antibody (aa54-75, clone 5-C3-1, Biotin) LS-C142008

Ordering

Wt. Vol. Conc. Price
- 100 µl 0.8 mg/ml $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CDKN2A / p16INK4a Antibodies

Anti-CDKN2A / p16INK4a Antibody (N-Terminus) IHC-plus™ LS-B1347
Rabbit Polyclonal (IgG) to Human CDKN2A / p16INK4a
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-CDKN2A / p16INK4a Antibody (clone 1E12E10) IHC-plus™ LS-B5261
Mouse Monoclonal [clone 1E12E10] (IgG1) to Human CDKN2A / p16INK4a
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CDKN2A / p16INK4a Antibody (aa50-150) LS-C49180
Rabbit Polyclonal to Mouse CDKN2A / p16INK4a
Mouse
IHC - Paraffin, IHC - Frozen, ICC, Western blot, ELISA
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rat Monoclonal [clone 5-C3-1] (IgG2b) to Mouse CDKN2A / p16INK4a
Mouse
IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry (applications tested for the base form of this product only)
Biotin Conjugated

Details

Mouse CDKN2A / p16INK4a
Rat
Mouse (tested or 100% immunogen sequence identity)
Human, Hamster
IgG2b Monoclonal [5-C3-1]
Biotin. Also available Unconjugated or conjugated with DY650, DY550.
Protein A purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin
  • IHC - Frozen (1:500 - 1:1000)
  • Immunofluorescence (1:500 - 1:1000)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:500 - 1:1000)
  • Flow Cytometry (1:500 - 1:1000)
  • (applications tested for the base form of this product only)

Specificity and Use

CDKN2A / p16INK4a antibody was raised against a synthetic peptide containing amino acids 54-75 of the murine p19ARF
aa54-75
Residues 62-75 of murine p19ARF.
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

PBS
Store at 4°C in the dark.
For research use only.

About CDKN2A / p16INK4a

P42771 NM_000077 NP_000068.1

CDKN2A Antibody, ARF Antibody, Cdk4 inhibitor Antibody, CDK4 inhibitor p16-INK4 Antibody, INK4 Antibody, INK4A Antibody, Multiple tumor suppressor 1 Antibody, MTS-1 Antibody, MTS1 Antibody, p16-INK4 Antibody, p16-INK4A Antibody, p16INK4 Antibody, p19 Antibody, p14 Antibody, p16 Antibody, p16 INK4A Antibody, p16INK4A Antibody, p14ARF Antibody, TP16 Antibody, CDK4I Antibody, CDKN2 Antibody, CMM2 Antibody, MLM Antibody, p19ARF Antibody

Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes.

Immunohistochemistry

Immunohistochemistry
Immunohistochemical staining of sections from a wild type mouse testis taken at P11 were probed with LS-C2744 (green). Sections were stained with DAPI to reveal the position of cell nuclei (blue). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Human Liver (formalin-fixed, paraffin-embedded) stained with CDKN2A antibody LS-B200 at 20 ug/ml followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunofluorescence

Immunofluorescence
Immunofluorescent detection of p19Arf using LS-C2744. Wild type mouse embryo fibroblasts (MEF) at passage 5 (panels 1, 2) and NIH3T3 cells, which have deleted the Arf gene (panels 3, 4) were probed with LS-C2744, followed by a fluorescently labeled anti-rat IgG secondary antibody (panels 1, 3). Cells were also stained with Hoechst dye to reveal the position of nuclei (panels 2, 4). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western blot analysis of endogenous p19Arf using LS-C2744. Total cell lysates (25 ug) from NIH3T3 cells, which have deleted the Arf gene (lane 1) and from wild type mouse embryo fibroblasts (MEFs) at passage 6, which express p19Arf (lane 2), were resolved by SDS-PAGE. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunoprecipitation

Immunoprecipitation
Immunoprecipitation of p19Arf using LS-C2744. Total cell lysates (250 ug) from NIH3T3 cells which have deleted the Arf gene (lane 5) and from NIH3T3 cells expressing an HA-tagged version of p19Arf (lanes 3, 4) were incubated with control rat IgG (lane 3), or LS-C2744 (lanes 4, 5) and protein G- Sepharose. Precipitated proteins were resolved by SDS-PAGE and analysed by Western blotting using an anti-HA antibody. Unprecipitated total cell lysates (25 ug, equivalent to 10% of immunoprecipitation) f. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/8/2016

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