LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us

CDKN1B / p27 Kip1 Antibody (phospho-Thr187) for WB/Western, IP, ELISA LS-C17123

P27 Kip1 antibody LS-C17123 is an unconjugated rabbit polyclonal antibody to human p27 Kip1 (CDKN1B). Validated for ELISA, IP and WB.
Catalog
Size
Price
LS-C17123-100
100 µg
$605
Description
P27 Kip1 antibody LS-C17123 is an unconjugated rabbit polyclonal antibody to human p27 Kip1 (CDKN1B). Validated for ELISA, IP and WB.
Target
Human CDKN1B / p27 Kip1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Western blot (5 - 10 µg/ml)
  • Immunoprecipitation
  • ELISA (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
Threonine187-phosphorylated p27 peptide derived from the C-terminus of the human p27 protein(1). This sequence differs by a single amino acid (not at the phosphorylated threonine) in the mouse, rat, and hamster p27 proteins.
Epitope
pThr187
Specificity
Specific for the Thr-187 phosphorylated form of the cyclin-dependent kinase inhibitor p27KIP1. The antibody does not react with unphosphorylated p27 or with related CDK inhibitor proteins. This antibody can be used to detect endogenous phosphorylated-p27 by Western blotting if lysates are first immunoprecipitated with 'plain' p27 antibody e. g. Rb x p27, cat no. 71-9600 or Ms x p27, cat no.33-2800). Characterization of this antibody has been carried out primarily with purified recombinant p27 incubated in kinase reactions.
Usage
Suitable for use in ELISA, Western Blot and Immunoprecipitation. ELISA: 0.1-1.0 ug/ml. Milk-derived blocking solutions reportedly contain phosphoproteins that may inhibit phosphoamino acid antibody binding and therefore should be avoided. A 3% BSA. solution may also be used. Immunoprecipitation: 15 ug/IP reaction. Western Blot: 5-10 ug/ml.
Presentation
PBS, pH 7.4, 0.05% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
About CDKN1B / p27 Kip1
P46527 NM_004064 NP_004055.1

Popular CDKN1B / p27 Kip1 Products

Anti-CDKN1B / p27 Kip1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Anti-CDKN1B / p27 Kip1 antibody IHC of human intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Anti-CDKN1B / p27 Kip1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-CDKN1B / p27 Kip1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Anti-CDKN1B / p27 Kip1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot

Publications (0)


Customer Reviews (0)


Requested From: United States
Date Requested: 1/19/2019
Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2019 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy