P27 Kip1 antibody LS-C17123 is an unconjugated rabbit polyclonal antibody to human p27 Kip1 (CDKN1B). Validated for ELISA, IP and WB.
Human CDKN1B / p27 Kip1
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
- Western blot (5 - 10 µg/ml)
- ELISA (0.1 - 1 µg/ml)
- (applications tested for the base form of this product only)
Threonine187-phosphorylated p27 peptide derived from the C-terminus of the human p27 protein(1). This sequence differs by a single amino acid (not at the phosphorylated threonine) in the mouse, rat, and hamster p27 proteins.
Specific for the Thr-187 phosphorylated form of the cyclin-dependent kinase inhibitor p27KIP1. The antibody does not react with unphosphorylated p27 or with related CDK inhibitor proteins. This antibody can be used to detect endogenous phosphorylated-p27 by Western blotting if lysates are first immunoprecipitated with 'plain' p27 antibody e. g. Rb x p27, cat no. 71-9600 or Ms x p27, cat no.33-2800). Characterization of this antibody has been carried out primarily with purified recombinant p27 incubated in kinase reactions.
Suitable for use in ELISA, Western Blot and Immunoprecipitation. ELISA: 0.1-1.0 ug/ml. Milk-derived blocking solutions reportedly contain phosphoproteins that may inhibit phosphoamino acid antibody binding and therefore should be avoided. A 3% BSA. solution may also be used. Immunoprecipitation: 15 ug/IP reaction. Western Blot: 5-10 ug/ml.
PBS, pH 7.4, 0.05% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.